标题:
农杆菌介导DR1372基因转化甘蓝型油菜
Preliminary Study on Agrobacterium-Mediated
Transformation of DR1372 Gene into Brassica napus L.
作者:
张思维, 周文波, 张新, 陈翠娜, 代其林, 王劲
关键字:
DR1372, 油菜, 农杆菌介导, 遗传转化
DR1372; Brassica napus L.; Genetic Transformation; Drought-Tolerance
期刊名称:
《Botanical Research》, Vol.2 No.1, 2013-01-25
摘要:
DR1372基因是在耐辐射奇球菌(Deinococcus radiodurans, DR)基因组中克隆得到的一个基因，其蛋白序列存在一个WHy功能域，此功能域可能参与油菜的抗旱过程。本研究首先构建植物DR1372- pBI121表达载体，然后利用农杆菌介导法将目的基因DR1372成功转入甘蓝型油菜中，并对影响油菜再生频率以及遗传转化效率的几个因素进行了探讨，初步建立了油菜转DR1372基因遗传转化体系，为DR1372基因的功能研究奠定了基础。The DR1372 containing a special domain called WHy was cloned from Deinococcus radiodurans. This WHy domain may be involved in the mechanism of drought resistance. In this study, the plant expression vector DR1372-pBI121 was constructed and introduced into Brassica napus L. mediated by Agrobacteria. Several factors affecting genetic transformation efficiency were explored and the Agrobacterium-mediated genetic transformation system was set up in Brassica napus. This study provides essential materials for later research on DR1372 gene.