标题:
厦门市售甲鱼蛋携带病原菌情况初步调查Primary Investigation of Pathogenic Bacteria from Sold Terrapin Eggs in Xiamen
作者:
吴玉娟, 孔繁德, 徐淑菲, 王仍瑞
关键字:
甲鱼蛋, MALDI-TOF-MS, 病原菌Terrapin Egg, MALDI-TOF-MS, Pathogen
期刊名称:
《Advances in Microbiology》, Vol.3 No.2, 2014-06-18
摘要:
目的：了解厦门市售甲鱼蛋携带病原菌情况。方法：按照病原菌的检测标准进行分离培养，获得纯培养物后，用基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)结合相应的诊断血清及PCR方法进行鉴定。结果：自7批厦门市售甲鱼蛋中分离到了16个属26种共76株细菌，甲鱼蛋带菌率为100%，多重带菌情况严重。结论：该次甲鱼蛋的检验结果表明，甲鱼蛋所携带病原菌种类广，大部分为多重带菌，部分携带致病菌或条件致病菌。因此必须做好甲鱼蛋的检疫工作，以确保人们身体健康和养殖业的健康持续发展。
Objective: To explore the situation of pathogens of Xiamenlocal terrapin eggs. Methods: The pathogens were isolated with standard methods. The pure cultures isolated from terrapin eggs were further specified by MALDI-TOF-MS, serological test and PCR methods. Results: 100% of 7 batches of Xiamen local terrapin eggs were contaminated with pathogenic bacteria according to MALDI- TOF-MS. 76 strains of isolated pathogenic bacteria fell into 16 genera and 26 species. All of the terrapin eggs carried five or more pathogenic bacteria. Conclusion: The results suggest that high- contamination rate, broad genera, coexistence of multiple species and isolation of pathogenic bacterium are very common phenomena. It’s very necessary to enforce surveillance of pathogenic bacteria in terrapin eggs.