标题:
房颤射频消融术可逆转冠状窦血miRNA的异常表达The radiofrequency ablation may reverse the abnormal miRNA expression in coronary sinus blood of patients with atrial fibrillation
作者:
赵楠楠, 徐桂玉, 杨水祥
关键字:
心房颤动, 射频消融, miRNA, 离子通道蛋白, 组学研究atrial fibrillation; radiofrequency ablation; miRNA; ion channel protein; genomics research
期刊名称:
《HANS Publication PrePrints》, Vol.1 No.1, 2016-02-14
摘要:
目的 探讨房颤射频消融手术（RFA）终止房颤后患者冠状窦血miRNA的变化，试图发现真正起调控作用的miRNA，以揭示房颤的机制及可能的干预靶点。方法 选择30例行房颤射频消融术患者（阵发性、持续性和永久性房颤各10例），健康体检者10例作为正常对照组。射频消融术前分别取冠状窦血和外周血，术后3个月取外周血，使用miRNA芯片进行全基因组miRNA表达谱微阵列分析，Real-time PCR对miRNA结果进行验证，并通过mirbase、miranda、targetscan数据库行靶基因分析，对重要miRNA进行双荧光素酶结合实验。结果 房颤射频消融术前患者冠状窦血与自身外周血比较，共有142种miRNA表达差异，其中6种显著上调，8种下调（p
Objective: To study the effect of radiofrequency ablation (RFA) on miRNA differential expression in coronary sinus blood (SB) of Atrial Fibrillation (AF) patients, and try to identify the possible pathogenesis of AF and miRNA targets for intervention in future. Methods: 30 AF patients (10 paroxymal, 10 persistent and 10 permanent AF patients) were enrolled, alongside 10 healthy subjects as controls. Peripheral blood (PB) samples were obtained before and at 3 months after RFA respectively. The total RNA was extracted and hybridized with the microRNA chips (microRNA v 18.0), and the differential expression of miRNA and clustering analysis in whole genome were made with Volcano Plot and tMEV software respectively, and validated by Real-time PCR. The target gene analysis of miRNAs was predicted through the Mirbase, Miranda and Targetscan databases. The important miRNAs were made for dual luciferase binding experiment to analyze and validate target genes.Results: There were 142 miRNAs differential expression in SB of pre-operation AF patients compared with PB of patient-self control, in which, 6 miRNAs were increased and 8 miRNAs were decreased significantly (P