标题:
分子生物学技术在土壤微生物多样性中的应用Molecular Biology Technology and Its Application in Soil Microbial Diversity
作者:
曹万举, 隋心, 韩士杰, 王云, 李金功, 王洁
关键字:
微生物多样性, 研究方法, 分子生物学, 生态系统Microbial Diversity; Research Method; Molecular Biology; Ecosystems
期刊名称:
《Bioprocess》, Vol.3 No.3, 2013-09-11
摘要:
微生物在生态系统中占据着很重要的作用，其结构和功能的多样性及变化在一定程度上反映了生态系统的基本状态。传统的微生物培养和鉴定方法得到的微生物信息很片面，不足以代表微生物在生态系统中的真实情况。近几十年发展起来的分子生物技术突破了传统方法的限制，极大地促进了微生物学和生态学的发展。本文介绍了现在常用的几种分子生物学方法在微生物多样性研究中的应用现状。
Microorganism plays a key role in ecosystem, the structure and function of microbial diversity, to a certain extent, reflects the status of ecosystem. It is unilateral to study microorganism with traditional cultural method, which could not reflect the real situation of microorganism in ecosystem. Recently, the molecular biology technology breaks the limitation of traditional cultural method and tremendously promotes the development of microbiology and ecology. So, this paper introduces several molecular biology methods which they’re applied in microbial diversity research.