标题:
利用叶绿体基因组高变片段对7个软枣猕猴桃居群遗传多样性的研究Study on Genetic Diversity of 7 Populations of Actinidia by Using Chloroplast Genome Fragment
作者:
刘虹, 刘锡红, 刘秋宇, 李刚, 覃瑞
关键字:
软枣猕猴桃, 叶绿体基因组, 高变片段, 系统进化树Actinidia arguta, Chloroplast Genome, Hypervariable Segment, Phylogenetic Tree
期刊名称:
《Botanical Research》, Vol.3 No.6, 2014-11-25
摘要:
软枣猕猴桃是猕猴桃属内倍性最复杂的物种之一，本研究针对分布在国内4个省份具有代表性的7个野生软枣猕猴桃居群，选用21对叶绿体基因组高变片段对7个居群的软枣猕猴桃进行序列比对、系统构建和多态性分析。研究发现，21对引物中有7对引物在7个软枣猕猴桃居群样本中均能扩增成功，第13号片段rpl32-trnL的分辨率最好。利用片段rpl32-trnL对7个软枣猕猴桃居群分别采用MP算法和NJ法构建的系统进化树表明，来自长白山的2个居群位于系统树最基部，来自大别山的3个居群并未处于同一支中，21个片段的平均多态位点数达到27.16。本研究为猕猴桃属叶绿体基因组的开发和应用奠定了基础。Actinidia arguta is one of the most complex species in the genus of Actinidia with different ploidy. In this paper, we choose seven representative wild populations of A. arguta in four provinces of China. We use 21 pairs of chloroplast genome hypervariable segments to analyse genetic diversity of seven populations. The result shows that seven pairs within twenty one pairs of primer fragment can amplify samples successfully. The No. 13 fragments rpl32-trnL had the best resolution ratio. We used the fragment rpl32-trnL to build the phylogenetic tree of seven populations with MP algorithm and NJ method. The experimental results show that two populations from Changbai Mountain are located at the base of the tree. Three populations from the Dabie Mountains are not in the same branch. At last, the average polymorphic locus of 21 fragments is 27.16. This study provided basis for the development and application of chloroplast genome in Actinidia species.