不同硒源对旧院黑鸡黑色素含量和抗氧化能力的影响

Effects of Different Selenium Sources on Melanin Content and Antioxidant Capacity of Jiuyuan Black Chicken

摘要: 通过在基础日粮中添加不同形态的硒源，探讨对旧院黑鸡血清中抗氧化酶活性、组织中黑色素和硒含量的影响。试验选择120只公母各半的5周龄旧院黑鸡，随机分为4组，每组3个重复，每个重复10只。4组分别为基础日粮组、添加亚硒酸钠组、添加蛋氨酸硒组和圈地散养组。添加亚硒酸钠组和添加蛋氨酸硒组的基础日粮中分别添加0.5 mg/kg不同形态的硒，试验周期为6周。结果表明，添加蛋氨酸硒组GSH-Px的活性极显著高于基础日粮组(P < 0.01)，圈地散养组GSH-Px的活性显著高于基础日粮组；SOD活性仅有添加蛋氨酸硒组才表现出一定的显著性；添加蛋氨酸硒组和圈地散养组T-AOC的抗氧化能力极显著高于基础日粮组(P < 0.01)，添加亚硒酸钠组T-AOC的抗氧化能力显著高于基础日粮组；添加蛋氨酸硒组MDA含量水平显示出显著降低，圈地散养组MDA含量水平显示出极显著降低(P < 0.01)；添加蛋氨酸硒组和圈地散养组的鸡胸肉和皮中黑色素的含量差异性均达到了极显著水平(P < 0.01)；添加蛋氨酸硒组和圈地散养组中鸡血清、鸡胸肉和鸡胸皮中硒含量水平均达到极显著水平(P < 0.01)。通过散养等方式养殖的旧院黑鸡，有机硒和黑色素含量较高，有较大的补硒和滋补养生价值。

Abstract: The effects of different forms of selenium sources in the basal diet on the activities of antioxidant enzyme, melanin and selenium in the serum of Jiuyuan black chicken were studied. A total of 120 5 weeks old Jiuyuan black chickens were randomly allotted into 4 groups with 3 replicates of 10 Chickens each. The 4 groups were as follows: basal diet, sodium selenite, methionine selenium and enclosure scatter-feed. The sodium selenite and methionine selenium groups were fed the basal diet supplemented with 0.5 mg/kg different forms selenium to 6 weeks of age, respectively. The results showed that activity of GSH-Px of the methionine selenium very significantly increased the basal diet; the activity of GSH-Px of the enclosure scatter-feed significantly increased the basal diet. SOD activity shows a certain significance only at the methionine selenium. The activity of T-AOC of the methionine selenium and enclosure scatter-feed very significantly increased the basal diet; the activity of T-AOC of the sodium selenite significantly increased the basal diet. The MDA content of the methionine selenium significantly decreased and the MDA content of the enclosure scatter-feed very significantly decreased. The difference of melanin content in chicken breast and skin reached very significant level in the methionine selenium and enclosure scatter-feed. The selenium of content in chicken serum, chicken breast and chicken breast skin reached a very significant level in the methionine selenium and enclosure scatter-feed. The enclosure scatter-feed Jiuyuan black chickens have higher content of organic selenium and melanin, which have great value of replenishing selenium and nourishing health.