中华被毛孢菌丝体对BALB/cByJ小鼠之非特异性免疫功能探讨

Effect of Hirsutella sinensis Mycelium on Non-Specific Immunity in BALB/cByJ Mice

摘要: 本研究目的为探讨中华被毛孢菌丝体对非特异性免疫功能影响，本实验将36只BALB/cByJ雌性(6周龄)小鼠随机分成三组：对照组(蒸馏水)、低剂量组与高剂量组(相当60公斤成人每天摄取1.59 g与15.9 g)，连续管喂中华被毛孢菌丝体五周后，分析小鼠脾脏细胞与腹腔巨噬细胞之非特异性免疫指标。实验结果发现中华被毛孢菌丝体对小鼠生长没有显著影响。在高剂量组其MHCI、CD4及CD8细胞数目显著较对照组增加(p < 0.05)，在MHC II、T、B及NK等淋巴细胞数目三组间无显著差异。高剂量组血清lgG及lgM抗体显著较对照组增加(p < 0.05)。在ConA，PHA及LPS三种裂殖素的分别刺激下，中华被毛孢菌丝体显著刺激脾脏淋巴细胞增生(p < 0.05)。在ConA刺激下，低剂量组显著刺激脾脏细胞产生Th1细胞激素IL-2及IFN-γ (p < 0.01)，高剂量组显著抑制脾脏细胞产生Th2细胞激素IL-4及IL-5 (p < 0.01)。在LPS刺激下，高剂量组显著刺激腹腔巨噬细胞生成IL-6 (p < 0.05)。中华被毛孢菌丝体具有促进B细胞免疫反应、调节Th1/Th2细胞激素分泌与促进巨噬细胞功能，显示中华被毛孢菌丝体具免疫调节功效。

Abstract: The aim of this study is to evaluate non-specific and specific immune responses on the Hirsutella sinensis mycelium. 36 BALB/cByJ female (6-week-old) mice were randomly divided into 3 groups and gave them commercial chow diet with supplementary Hirsutella sinensis mycelium by oral administration of control (distilled water), low and high dosages (equivalent to 1.59, 15.9 g/60 kg/day for adult). After 5 weeks’ experimental treatment, BALB/cByJ mice were sacri-ficed to evaluate the secretion on cytokine and antibody from spleen cell and peritoneal macro-phages of mice. The result of the non-specific immune study showed that two experimental groups had no significant effect on their growth compared with control group. The number of MHCI, T, CD4, and CD8 lymphocytes in the high dosage group was significantly higher than that in the control group (p < 0.05). There was no significant difference among the three groups’ lymphocytes such as MHC II, B, and NK. The high dosage group significantly increased in serum immunoglobulin G (IgG) and IgM than that in the control group (p < 0.05). Splenocyte prolifera-tion significantly increased in low and high dosage groups compared with control group by concanavalin A (ConA), Phytohemagglutinin (PHA) and lipopolysaccharide (LPS) stimulating (p < 0.05). Compared with control group by ConA stimulating, we observed that low dosages signif-icantly promoted the secretions of IL-2 and IFN-γ (p <0.01), but the high dosages significantly inhibited the secretions of IL-4 and IL-5 (p < 0.01). The high dosage group significantly stimu-lated the IL-6 production in peritoneal macrophages compared with control group by LPS stim-ulating (p < 0.05). These results showed that the Hirsutella sinensis mycelium improved non-specific immunity in BALB/cByJ mice.