大庆产中药与血清相互作用的荧光光谱研究

Study on the Fluorescence Spectra of Interactions between Serum and Chinese Medicine Produced in Daqing

摘要: 利用RF5301荧光分光光度计研究了大庆产中药防风与不同生化指标的人体血清相互作用的荧光光谱。实验结果表明：大庆防风的荧光区域主要分布在320~570 nm，其最佳激发波长为310 nm，在428 nm处有荧光峰，荧光强度最大。血清在加入大庆防风后的荧光峰形状不发生改变，但荧光范围缩小(300~450 nm)。大庆防风对不同生化指标血清的荧光猝灭程度不同，其中对正常和高血脂血清荧光猝灭作用最强，对高血糖血清猝灭程度较弱。本研究为中草药与血清的光谱特性研究提供了一定的参考价值。

Abstract: The fluorescence spectra of serum interaction between Daqing Saposhnikovia divaricata and dif-ferent biochemical indexes were studied by RF5301 fluorescence spectrophotometer. The results showed that the fluorescence of Daqing Saposhnikovia divaricata was mainly distributed in the re-gion of 320~570 nm; the best excitation wavelength is at 310 nm; the fluorescence peak position is at 428 nm. The spectrum shape of serum remained unchanged after adding Saposhnikovia divari-cata, but the range of luminescence is reduced (300~450 nm). The fluorescence quenching degree of the serum of Daqing Saposhnikovia divaricata against different biochemical indexes was different, and the fluorescence quenching effect of the normal and hyperlipemia serum was the strongest, while the hyperglycemia was weak. This study provides a certain reference value for the study of spectral characteristics of Chinese herbal medicine and serum.