响应面分析法优化六堡茶茶多糖提取工艺的研究

Study on the Extracting Conditions of Liupao Tea Polysaccharides Based on Response Surface Methodology

摘要: 目的：优化六堡茶茶多糖的提取工艺，寻找最优方案，减少提取操作的盲目性，为进一步的试验研究提供参考。方法：以响应面分析法考察热水浸提提取茶多糖试验，在单因素试验的前提下，选择液料比、提取时间、提取温度为自变量，以茶多糖的提取率为响应值，根据Box-Benhnken中心组合试验与响应面分析法，研究各个自变量的交互作用与其对茶多糖提取率的影响，模拟得出二次多项式回归方程预测模型，从而确定茶多糖提取工艺的最佳条件。结果：六堡茶茶多糖提取工艺的最佳条件为料液比1:17，提取时间83 min，提取温度93℃。在此条件下，六堡茶茶多糖的提取率达到8.0%。结论：采用响应面分析法对六堡茶茶多糖提取条件的优化是可行的。

Abstract: Objective: To optimize the Liupao tea’s polysaccharide extraction process, reduce the blindness of extraction operation, and provide reference for further experimental study. Methods: Response surface methodology is used to study extraction of Liupao Tea’s polysaccharide. On the basis of single-factor test, the mathematical regression model is established on the independent variables (ratio of water to material, extraction time and extraction temperature) and response value (extraction yield of tea polysaccharides) through Box-Benhnken Center combination test and Response Surface Methodology. The various independent variables’ interaction and its influence on tea’s polysaccharide extraction yield, simulation for quadratic polynomial regression equation prediction model are studied, and the best conditions of tea’s polysaccharide extraction process are concluded. Results: The best conditions of tea’s polysaccharide extraction process are: material to water ratio 1:17, time 83 min, extraction temperature 93˚C. Under these conditions, the yield of tea polysaccharide is 8.0%. Conclusions: Response surface methodology is feasible to optimize the extraction conditions of Liupao Tea polysaccharides, and can provide reference for further experimental study.