罗汉果性别决定机制的分析
The Analysis of the Sex Determination Mechanism in Monk Fruit
DOI: 10.12677/hjfns.2025.146090, PDF,   
作者: 尹小明*：大闽食品(漳州)有限公司，福建 漳州；上海交通大学药学院，上海；上海吉罗恩生物科技有限公司，上海；高艺航, 阮文浩, 张 玺：大闽食品(漳州)有限公司，福建 漳州；上海吉罗恩生物科技有限公司，上海；福建省饮料用植物提取技术企业重点实验室，福建 漳州；黄清坤：大闽食品(漳州)有限公司，福建 漳州；福建省饮料用植物提取技术企业重点实验室，福建 漳州
关键词: 罗汉果；分子标记；性别决定；雌雄鉴定；Monk Fruit； Molecular Marker； Sex Determination； Sex Identification
摘要: 罗汉果(Siraitia grosvenorii)，作为中国广西的特色经济物种，不仅具有很高的药用价值，还富含高甜味且零热量的天然甜苷成分。罗汉果属于葫芦科雌雄异株的植物，其性别在苗期难以鉴别。DNA分子标记是个体间遗传物质内核苷酸序列变异为基础的遗传标记，DNA分子标记技术为罗汉果雌雄的有效鉴定提供了手段。本文收集了已报道罗汉果中与性别相关的DNA分子标记，并将这些DNA分子标记定位到了基因组的重叠群和可能的基因上。此外，我们还对这些基因进行了同源比对分析，预测了其可能的编码产物，这为研究罗汉果性别决定机制提供了重要的研究基础。
Abstract: Monk fruit (Siraitia grosvenorii), a characteristic economic species of Guangxi, China, not only possesses substantial medicinal value but is also rich in high-intensity, zero-calorie natural sweet glycosides (mogrosides). As a dioecious member of the Cucurbitaceae, monk fruit exhibits sexes that are difficult to distinguish at the seedling stage. DNA molecular markers—genetic markers based on nucleotide sequence variation among individuals—provide an approach for reliable sex identification in this species. In the present study, we collected previously reported DNA molecular markers associated with sex in monk fruit and mapped these markers to genomic contigs and candidate genes. We further performed homology-based comparative analyses of these genes and predicted their putative protein products, thereby providing an important foundation for future research into the sex-determination mechanism of monk fruit.
文章引用：尹小明, 高艺航, 阮文浩, 张玺, 黄清坤. 罗汉果性别决定机制的分析[J]. 食品与营养科学, 2025, 14(6): 822-830. https://doi.org/10.12677/hjfns.2025.146090

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