HBV相关慢加急性肝衰竭患者肠道菌群研究

Investigation on Intestinal Flora in Patients with HBV-Associated Acute Chronic Liver Failure

摘要: 探讨慢加急性肝衰竭患者肠道菌群富集以及变化情况，寻找肠道菌群与慢加急性肝衰竭重症化关联。方法：本研究所纳入新疆医科大学第一附属医院住院的患者。实验分为3组，第一组肝硬化组40人，第二组慢加急性肝衰竭9人，健康对照20人。提取粪便样本的DNA，运用16rDNA的V3-V4区高通量测序方法分析研究肠道菌群的结构、丰度及多样性等特点，通过对HBV-ACLF患者肠道菌群与慢性肝衰竭及健康人群的肠道菌群进行对比，以寻找出与HBV-ACLF发生发展相关的菌群。结果：实验组相较于对照组差异OTU主要以上调为主，门水平上表现在厚壁菌门、变形菌门，科水平上主要表现在乳杆菌科、链球菌科及韦荣氏菌科；我们使用a多样性和β多样性分析了三组之间的物种多样性差异。与肝硬化组、健康组相比，慢加急性肝衰竭组的肠道微生物群落的丰度逐渐降低，但是肠道微生物的多样性差异不显著。

Abstract: Objective: Explore the enrichment and changes of gut microbiota in patients with chronic acute liver failure, and search for the association between gut microbiota and the severity of chronic acute liver failure. Method: This study included hospitalized patients at the First Affiliated Hospital of Xinjiang Medical University. The experiment was divided into three groups: the first group consisted of 40 people with cirrhosis, the second group consisted of 9 people with chronic and acute liver failure, and the healthy control group consisted of 20 people. Extract DNA from fecal samples and analyze the structure, abundance, and diversity of gut microbiota using high-throughput sequencing of the V3-V4 region of 16rDNA. By comparing the gut microbiota of HBV-ACLF patients with chronic liver failure and healthy individuals, we aim to identify the microbiota associated with the occurrence and development of HBV-ACLF. Result: Compared with the control group, the difference in OTU between the experimental group and the control group was mainly upregulated. At the phylum level, it was manifested in the phyla Firmicutes and Proteobacteria, and at the family level, it was mainly manifested in the families Lactobacillus, Streptococcus, and Veillonella; we analyzed the differences in species diversity among the three groups using alpha diversity and beta diversity. Compared with the cirrhosis group and the healthy group, the abundance of gut microbiota in the chronic acute liver failure group gradually decreased, but the diversity of gut microbiota was not significantly different.