液相色谱–串联质谱法测定化妆品中7种小分子肽含量

Determination of 7 Kinds of Small Molecular Peptides in Cosmetics by HPLC-MS/MS

摘要: 建立了液相色谱–串联质谱法测定化妆品中肌肽等7种小分子肽含量的方法。样品采用1%氯化钠水溶液:甲醇:乙酸 = 6:3:1混合溶剂超声提取，液相分离，质谱外标法定量。结果表明，7种小分子肽在各自范围内线性关系良好(相关系数 > 0.996)，检出限在0.05~0.5 μg/L之间，在低、中、高三个添加水平级别，加标回收率在82.7%~109.5%之间，RSD在1.1%~6.7%之间。该方法前处理简单高效，专属性强，灵敏度高，准确性好，可用于化妆品中7种小分子肽的筛查和含量测定。

Abstract: A HPLC-MS/MS method was developed for the determination of 7 small molecular peptides in cosmetics. The samples were extracted with 1% NaCl solution: methanol: acetic acid = 6:3:1 mixed solvent by ultrasonic, separated by liquid phase, and quantified by external standard mass spectrometry. The results showed that the linear relationship of the 7 peptides was good (correlation coefficient > 0.996), and the detection limit was between 0.05~0.5 μg/l, the recoveries ranged from 82.7% to 109.5%, and the RSD ranged from 1.1% to 6.7%. The method is simple and efficient with high specificity, high sensitivity and accuracy. It can be used for screening and determination of 7 small molecular peptides in cosmetics.