结直肠癌组织中COL1A1、miR-143的表达水平和临床意义以及两者的相关性

The Expression Levels, Clinical Significance, and Correlation of COL1A1 and miR-143 in Colorectal Cancer Tissues

摘要: 目的：探讨COL1A1和miR-143在结直肠癌中的表达水平和临床意义以及两者的相关性。方法：收集80例结直肠癌患者的组织蜡块和50例结直肠癌患者的新鲜标本，分别用IHC和qRT-PCR检测COL1A1和miR-143的表达水平，并利用统计学方法分析两者表达水平的相关性及与患者临床病理参数的关系。结果：在结直肠癌组织中，COL1A1的表达高于癌旁组织(P < 0.001)，miR-143的表达低于癌旁组织(P < 0.001)，两者的相对表达量呈显著负相关且都与肿瘤直径、分化程度、淋巴结转移、TNM分期相关(P值 < 0.05)；结论：1) 在结直肠癌组织中，COL1A1的表达升高，miR-143的表达降低，且miR-143表达低时，COL1A1mRNA表达高，反之亦然；2) 两者表达水平都与肿瘤的直径、分化程度、淋巴结转移和TNM分期相关。

Abstract: Objective: To investigate the expression level and clinical significance of COL1A1 and miR-143 in colorectal cancer and their correlation. Methods: Collect tissue wax blocks from 80 patients with colorectal cancer and fresh specimens from 50 patients with colorectal cancer. The expression levels of COL1A1 and miR-143 were detected by IHC and qRT-PCR, respectively, and the correlation between the expression levels of the two genes and their relationship with clinical pathological parameters were analyzed using statistical methods. Results: In colorectal cancer tissues, the expression of COL1A1 was higher than that of adjacent tissues (P < 0.001), and the expression of miR-143 was lower than that of adjacent tissues (P < 0.001). The relative expression levels of the two were significantly negatively correlated. All are related to tumor diameter, degree of differentiation, lymph node metastasis, TNM staging (P value < 0.05); Conclusion: 1) In colorectal cancer tissues, the expression of COL1A1 increases, the expression of miR-143 decreases, and when the expression of miR-143 is low, COL1A1mRNA expression is high, and vice versa; 2) Both expression levels are related to tumor diameter, degree of differentiation, lymph node metastasis and TNM stage.