ACAT1 rs11545566多态性与非酒精性脂肪性肝病易感性的相关性

Association between ACAT1 rs11545566 Polymorphism and Susceptibility to Non-Alcoholic Fatty Liver Disease

摘要: 目的：探究中国青岛地区乙酰辅酶A乙酰转移酶1 (acetyl-CoA acetyltransferase 1, ACAT1)基因rs11545566位点多态性与非酒精性脂肪性肝病(non-alcoholic fatty liver disease, NAFLD)易感性的相关性。方法：纳入于青岛市市立医院收集的NAFLD患者226例，健康对照141例。收集所有受试者血液标本，提取DNA，通过聚合酶链反应的方法扩增DNA，并检测ACAT1基因rs11545566位点的基因型。收集并分析患者的临床资料及实验室指标。符合正态分布的计量资料采用t检验，不符合正态分布的计量资料采用Wilcoxon秩和检验进行组间比较，使用χ²检验分析NAFLD和对照组的基因型及等位基因分布差异。结果：ACAT1 rs11545566位点在NAFLD组和对照组的基因型与等位基因分布差异均无统计学意义(P > 0.05)。ACAT1 rs11545566位点G等位基因携带者比非携带者ALT水平更低(P = 0.038)。结论：在中国青岛汉族人群中，ACAT1 rs11545566位点多态性与NAFLD易感性无显著相关性。ACAT1 rs11545566 位点G等位基因携带可能与低ALT水平有关。

Abstract: Objective: To explore the polymorphism of acetyl-CoA acetyltransferase 1 (ACAT1) rs11545566 locus and non-alcoholic fatty liver disease in Qingdao region of China association with susceptibility to NAFLD. Methods: 226 patients with NAFLD and 141 healthy controls admitted to Qingdao Municipal Hospital were included. Blood samples were collected from all subjects and DNA was extracted. Then DNA was amplified by polymerase chain reaction and we genotyped the rs11545566 locus of the ACAT1 gene. Clinical data and laboratory indicators were collected and analyzed. T-test was used for measurement data conforming to normal distribution, and Wilcoxon rank sum test was used for comparison between groups for measurement data not conforming to normal distribution. χ² test was used to analyze the differences in genotype and allele distribution between NAFLD and control group. Results: There were no significant differences in genotype and allele distribution of ACAT1 rs11545566 between NAFLD group and control group (P > 0.05). ACAT1 rs11545566 G allele carriers have lower ALT levels than non-carriers (P = 0.038). Conclusion: There is no significant correlation between ACAT1 rs11545566 polymorphism and NAFLD in Qingdao Han population. ACAT1 rs11545566 G allele is associated with lower levels of ALT.