EICP修复Cu²⁺污染液的影响因素

The Influence Factors of Remediation of Cu²⁺ Contaminated Solution by EICP

摘要: 我国铜污染问题日益严峻，亟需环保高效的修复方法予以处理。脲酶诱导碳酸盐沉淀(Enzyme Induced Carbonate Precipitation, EICP)技术作为新型生物修复技术在重金属修复领域被广泛关注，但是对特定重金属污染(例如铜、镉等)的修复研究匮乏。文章采用EICP技术开展了一系列Cu²⁺污染液修复的试验研究，通过pH值、Cu²⁺修复率、Ca²⁺沉淀率、总沉淀量等修复指标，探究了EICP技术对Cu²⁺污染液的修复效果；分析了Cu²⁺污染液中Cu²⁺初始浓度、氯化钙浓度、脲酶提取液体积、修复时间等因素对修复效果的影响规律。结果表明：在一定条件下，EICP技术对Cu²⁺污染液的修复率最高可达40.03%；降低Cu²⁺初始浓度、增加脲酶提取液体积、适当提高氯化钙浓度、控制修复时间等均能促进铜离子污染的修复反应，其中污染液的Cu²⁺初始浓度为600 mg/L、脲酶提取液体积为20 mL、氯化钙浓度为1.6 mol/L、修复时间为12 h时，铜离子污染的修复效果更好，经济性更优。

Abstract: The copper pollution problem is becoming more and more serious in our country, which urgently needs environmental and efficient remediation methods to deal with it. Although the Enzyme Induced Carbonate Precipitation (EICP) technology has gained significant attention in the field of heavy metal remediation as a novel bioremediation technique, research on the remediation of specific heavy metal contaminants such as copper and cadmium are still deficient. A series of experimental studies were conducted in this paper utilizing EICP technology to remediate Cu²⁺ contaminated solution. The remediation efficiency of EICP on Cu²⁺ contaminated solution was investigated through remediation indicators, including pH value, Cu²⁺ removal ratio, Ca²⁺ precipitation ratio, and total precipitation amount. Additionally, the influence of factors such as the initial Cu²⁺ concentration in the contaminated solution, calcium chloride concentration, volume of urease extract, and remediation duration on the remediation efficiency was analyzed. The results indicate that under certain conditions, the Cu²⁺ removal ratio from contaminated solutions by EICP can reach a maximum value of 40.03%. Lowering the initial Cu²⁺ concentration, increasing the volume of urease extract, moderately enhancing the calcium chloride concentration, and controlling the remediation duration facilitate the remediation reaction of copper ion pollution. Specifically, the optimal remediation and better economy are found with the combination of 600 mg/L initial Cu²⁺ concentration in the contaminated solution, 20 mL urease extract, 1.6 mol/L calcium chloride concentration, and 12 hours remediation duration.