微波消解–原子荧光法测定夏蝉总汞检测

Detection of Total Mercury by Microwave Digestion-Atomic Fluorescence Method

摘要: 本研究采用微波消解–原子荧光法测定夏蝉体内汞的含量。5 g夏蝉样品经过微波消解后，载流为5%的硝酸溶液，还原剂为0.5%氢氧化钾+0.01%硼氢化钠，利用原子荧光光度计检测。结果显示，检出限为0.045 μg·L⁻¹，R² ≥ 0.9998，回收率为84.2%~116.40%，RSD ≤ 2.15%，所得出的汞标准曲线呈现良好的线性关系。该方法准确、可靠，适用于夏蝉体内汞含量检测。

Abstract: This study utilized microwave digestion-atomic fluorescence spectrometry to determine the mercury content in Cicada (summer cicada) samples. After microwave digestion of 5g cicada samples, a 5% nitric acid solution was used as the carrier, and a reducing agent mixture of 0.5% potassium hydroxide and 0.01% sodium borohydride was applied. Detection was performed using an atomic fluorescence spectrophotometer. The results showed a detection limit of 0.045 μg·L⁻¹, with R² ≥ 0.9998. The recovery rate ranged from 84.2% to 116.40%, and the relative standard deviation (RSD) was less than or equal to 2.15%. The mercury standard curve exhibited good linearity. This method is accurate and reliable, making it suitable for detecting mercury content in cicadas.