Egr1和Fos基因在手术后应用羟考酮麻醉中发挥巨大作用

Role of Egr1 and Fos Genes in Oxycodone Anesthesia Application Post-Surgery

摘要: 背景：Egr1、Fos基因在手术后应用羟考酮麻醉中的作用尚不清楚。方法：羟考酮麻醉数据集GSE232804配置文件是从GPL20084生成的基因表达综合(GEO)数据库中下载的。进行差异表达基因(DEGs)的筛选，加权基因共表达网络分析(WGCNA)，功能富集分析，蛋白质–蛋白质相互作用(PPI)网络的构建与分析。绘制基因表达量热图。通过毒理学基因组学比较数据库(CTD)寻找与核心基因的最相关的疾病。TargetScan用于筛选调节中枢DEG的miRNA。结果：共得到了171个DEGs。根据基因本体论(GO)分析，在BP分析中，它们主要富集在细胞对促肾上腺皮质激素释放激素刺激的反应、神经元分化、神经元突触可塑性的调节。在CC分析中，它们主要富集在核、转录因子复合体、神经元胞体。在MF分析中，它们主要集中在蛋白结合、转录辅抑制因子活性、蛋白激酶活性。在KEGG分析中，它们主要富集在MAPK信号通路、安非他命上瘾、nf-κB信号通路、TNF信号通路。PPI网络中获得了核心基因(Rasl11a、Map3k14、Dusp5、Dusp6、Arl4d、Kdm6b、Egr1、Egr3、Bcorl1)。WGCNA分析中的软阈值功率设置为9，一共生成了8个模块。最终获得了5个核心基因(Dusp1、Egr1、Egr2、Fos、Nr4a1)。核心基因表达量热图发现核心基因(Dusp1、Egr1、Egr2、Fos、Nr4a1)在术后应用羟考酮麻醉样本中均为高表达。CTD分析发现核心基因(Dusp1、Egr1、Egr2、Fos、Nr4a1)和免疫系统疾病、神经系统疾病、心律失常、心动过速、消化系统异常有关。结论：Egr1、Fos在术后应用羟考酮麻醉样本中高表达。

Abstract: Background: The role of Egr1 and Fos genes in oxycodone anesthesia post-surgery remains unclear. Methods: The oxycodone anesthesia dataset GSE232804 profile was downloaded from the GEO database, generated by GPL20084. Differentially expressed genes (DEGs) were screened, and weighted gene co-expression network analysis (WGCNA), functional enrichment analysis, and protein-protein interaction (PPI) network construction and analysis were conducted. A heatmap of gene expression was generated. The Comparative Toxicogenomics Database (CTD) was used to identify diseases most related to core genes, and TargetScan was used to screen miRNAs regulating central DEGs. Results: A total of 171 DEGs were identified. GO analysis showed that in the BP category, they were primarily enriched in cellular response to corticotropin-releasing hormone, neuron differentiation, and regulation of synaptic plasticity. In the CC category, they were mainly enriched in the nucleus, transcription factor complex, and neuron cell body. In the MF category, they were focused on protein binding, transcription corepressor activity, and protein kinase activity. KEGG analysis showed enrichment in the MAPK signaling pathway, amphetamine addiction, NF-kappa B signaling pathway, and TNF signaling pathway. Core genes (Rasl11a, Map3k14, Dusp5, Dusp6, Arl4d, Kdm6b, Egr1, Egr3, Bcorl1) were identified in the PPI network. The soft-thresholding power in WGCNA analysis was set to 9, generating a total of eight modules. Five core genes (Dusp1, Egr1, Egr2, Fos, Nr4a1) were identified. The heatmap of core gene expression showed that Dusp1, Egr1, Egr2, Fos, and Nr4a1 were highly expressed in samples of oxycodone anesthesia post-surgery. CTD analysis revealed that the core genes (Dusp1, Egr1, Egr2, Fos, Nr4a1) were associated with immune system diseases, neurological diseases, arrhythmia, tachycardia, and digestive system abnormalities. Conclusion: Egr1 and Fos are highly expressed in oxycodone anesthesia samples post-surgery.