覆盆子多酚的大孔树脂富集工艺优化及生物活性评价

Optimization of Macroporous Resin Enrichment Process and Determination of Bioactivity of Polyphenols from Rubus chingii Hu

摘要: 研究覆盆子多酚的大孔树脂富集工艺，并评价覆盆子多酚的生物活性。通过静态吸附–解吸试验选择适合富集覆盆子多酚的大孔树脂，拟合吸附方程判断大孔树脂的吸附动力学模型，以大孔树脂对覆盆子多酚粗提物中多酚的吸附量和解吸率为指标，对大孔树脂的上样液pH、多酚上样量、解吸液乙醇体积分数进行单因素试验，并通过正交试验确定大孔树脂富集覆盆子多酚的最优条件组合；评价富集后覆盆子多酚的纯度、对1,1-二苯-2-苦肼基(DPPH·)清除率以及对黄嘌呤氧化酶和α-葡萄糖苷酶的抑制活性，通过分子对接评价覆盆子主成分鞣花酸与黄嘌呤氧化酶、α-葡萄糖苷酶的结合能力。结果显示，AB-8大孔树脂最适合富集覆盆子多酚且其吸附覆盆子多酚的过程符合准二阶动力学模型，AB-8大孔树脂在上样液pH为3、多酚上样量为30 mg/g树脂、解吸液的乙醇体积分数为90%时，制备的覆盆子多酚的得率最高，达到85.36 ± 0.78%，富集后覆盆子多酚纯度从23.81 ± 1.75%上升到68.58 ± 0.60%，对DPPH·的清除率以及对黄嘌呤氧化酶和α-葡萄糖苷酶的抑制率的IC₅₀分别为59.09 ± 0.42 μg/mL、97.71 ± 2.75 μg/mL、3.98 ± 0.43 μg/mL，分子对接结果也显示鞣花酸化合物与黄嘌呤氧化酶和α-葡萄糖苷酶有较好的结合能力。本研究为覆盆子多酚的工业化生产和开发利用提供理论参考。

Abstract: The study was conducted to optimize the adsorption process of polyphenols from raspberry (Rubus chingii Hu) on macroporous resin and evaluate the biological activity of polyphenols from raspberry . The macroporous resin suitable for adsorbing raspberry polyphenols was selected through static adsorption-desorption experiments, and the adsorption kinetic model was determined by fitting the adsorption equation. The adsorption capacity and desorption rate of polyphenols in crude extract of raspberry polyphenols by macroporous resin were used as indicators, and the pH of sample solution, the amount of polyphenol sample, and the ethanol volume fraction of desorption solution were tested by single factor test. The optimal combination of polyphenol enrichment conditions was determined by orthogonal test. The purity, DPPH· scavenging rate, and inhibition rates of xanthine oxidase and α-glucosidase of the polyphenols from raspberry adsorbed by macroporous resin were evaluated. The binding ability of ellagic acid, the main component of raspberry, to xanthine oxidase and α-glucosidase was evaluated by molecular docking. The results showed that AB-8 macroporous resin was the most suitable for adsorbing polyphenols from raspberry, and the adsorption process of polyphenols from raspberry on AB-8 macroporous resin conformed to the quasi-second-order kinetic model. When the pH of the feed solution was 3, the loading amount of polyphenols was 30 mg/g of resin, and the ethanol content of the elution solution was 90%, the yield of polyphenols from raspberry prepared by AB-8 macroporous resin was the highest, reaching 85.36 ± 0.78%. The purity of polyphenols from raspberry adsorbed by AB-8 macroporous resin increased from 23.81 ± 1.75% to 68.58 ± 0.60%, the DPPH· scavenging rate was 59.09 ± 0.42 μg/mL, and the inhibition rates of xanthine oxidase and α-glucosidase were 97.71 ± 2.75 μg/mL and 3.98 ± 0.43 μg/mL, respectively. Molecular docking results also showed that ellagic compounds had good binding ability to xanthine oxidase and α-glucosidase. This study provides theoretical reference for the industrialization production and development and utilization of polyphenols from raspberry.