Alisol A抑制鼻咽癌细胞的增殖、迁移和侵袭
Alisol A Inhibited the Proliferation, Migration, and Invasion of Nasopharyngeal Carcinoma Cells
DOI: 10.12677/acm.2025.151295, PDF,   
作者: 刘会清, 张艺璇：河北大学附属医院耳鼻喉科，河北 保定；刘海燕*：河北大学附属医院神经内科，河北 保定
关键词: Alisol A；鼻咽癌；Alisol A； Nasopharyngeal Carcinoma
摘要: 目的：Alisol A是从泽泻中分离得到的具有生物活性的三萜类化合物，具有抗癌潜能。本研究旨在探讨Alisol A对鼻咽癌细胞生长的影响。方法：MTT实验、集落形成实验、流式细胞术、transwell实验、伤口愈合实验分别检测细胞活力、增殖、细胞周期、迁移、侵袭。结果：Alisol A可抑制鼻咽癌细胞的活力、增殖、迁移和侵袭。Alisol A对C666-1和HK1细胞的生长有明显的抑制作用，并呈时间和浓度依赖性。Alisol A处理显著降低了鼻咽癌细胞中细胞周期相关基因的蛋白表达。在Alisol A处理的细胞中，鼻咽癌细胞的迁移和侵袭能力降低。Alisol A处理显著降低了鼻咽癌细胞中MMP2和MMP9的蛋白表达。结论：Alisol A抑制鼻咽癌细胞的增殖、迁移和侵袭。Alisol A可能是治疗鼻咽癌的靶点。
Abstract: Purpose: Alisol A is a bioactive triterpenoid isolated from the Rhizoma alismatis, which has anticancer potential. In this study, we explored the effect of Alisol A on the growth of nasopharyngeal carcinoma (NPC) cells. Methods: MTT assay, colony formation assay, flow cytometry, transwell assay, wound healing assay were used to assess cell viability, proliferation, cell cycle, migration, invasion, respectively, in vitro. Results: Alisol A inhibited the viability, proliferation, migration, and invasion of NPC cells. Alisol A significantly inhibited the growth of C666-1 and HK1 cells in a time- and concentration-dependent manner. Alisol A treatment significantly reduced the protein expression of cell cycle-related genes in NPC cells. The migration and invasion abilities of NPC cells were reduced in Alisol A-treated cells. Alisol A treatment significantly reduced the protein expression of MMP2 and MMP9 in NPC cells. Conclusion: Alisol A inhibited the proliferation, migration, and invasion of NPC cells. Alisol A may be a potential therapeutic target for nasopharyngeal.
文章引用：刘会清, 张艺璇, 刘海燕. Alisol A抑制鼻咽癌细胞的增殖、迁移和侵袭[J]. 临床医学进展, 2025, 15(1): 2250-2258. https://doi.org/10.12677/acm.2025.151295

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