妊娠期糖尿病患者血清Pannexin-1表达与胰岛素抵抗相关性分析

Correlation Analysis of Serum Pannexin-1 Expression and Insulin Resistance in Patients with Gestational Diabetes Mellitus

摘要: 目的：探讨妊娠期糖尿病(GDM)患者外周血中Pannexin-1 (PANX1)表达与胰岛素抵抗的相关性分析。方法：选取2023年12月至2024年11月在青岛大学附属医院产科住院分娩的30例GDM患者作为实验组，同时选取30例正常妊娠孕妇作为对照组。收集两组孕妇的外周血，采用酶联免疫吸附试验(ELISA)检测外周血中PANX1的浓度。使用稳态模型评估胰岛素抵抗指数(HOMA-IR)及胰岛素抵抗情况，并进行相关性分析。结果：GDM组与对照组在年龄、孕周、孕前BMI等一般资料无显著差异(P > 0.05)，空腹血糖(FBG)、空腹胰岛素(FINS)和HOMA-IR，GDM组显著高于对照组(P < 0.05)。GDM患者外周血中PANX1的浓度显著高于对照组(184.4 ± 106.03 pg/mL vs. 87.34 ± 2.68 pg/mL, P < 0.001)。PANX1水平与HOMA-IR呈正相关(P < 0.05)。结论：GDM患者外周血中PANX1水平的升高与胰岛素抵抗的增加有关，PANX1可能在GDM的发病机制中发挥重要作用。

Abstract: Objective: To investigate the correlation between the expression of Pannexin-1 (PANX1) in the peripheral blood of patients with gestational diabetes mellitus (GDM) and insulin resistance. Methods: This study included 30 pregnant women diagnosed with GDM who delivered at the Affiliated Hospital of Qingdao University from December 2023 to November 2024 as the experimental group, and 30 healthy pregnant women as controls. Peripheral blood samples were collected from both groups. The concentration of PANX1 in peripheral blood was measured using enzyme-linked immunosorbent assay (ELISA). Insulin resistance was assessed using the homeostasis model assessment of insulin resistance (HOMA-IR), and correlation analysis was performed. Results: There were no significant differences between the GDM and control groups in terms of age, gestational age, or pre-pregnancy BMI (P > 0.05). However, the GDM group had significantly higher levels of fasting blood glucose (FBG), fasting insulin (FINS), and HOMA-IR compared to the control group (P < 0.05). The concentration of PANX1 in the peripheral blood of GDM patients was significantly higher than that of the controls (184.4 ± 106.03 pg/mL vs. 87.34 ± 2.68 pg/mL, P < 0.001). PANX1 levels were positively correlated with HOMA-IR (P < 0.05). Conclusion: The elevated levels of PANX1 in the peripheral blood of GDM patients are associated with increased insulin resistance, suggesting that PANX1 may play an important role in the pathogenesis of GDM.