35份辣椒种质资源辣椒素类物质和辣椒红素的精准评价

Precise Evaluation of Capsaicinoids and Capsanthin in 35 Pepper Germplasm Resources

摘要: 为筛选高辣高红色素的优异辣椒种质，本研究以35份辣椒种质资源作为实验材料，运用高效液相色谱(HPLC)技术，开展辣椒素类物质及辣椒红素含量的精准评价。检测方法呈现出良好线性关系，确保分析结果准确可靠。35份辣椒种质辣椒素类物质含量方面，热辣6号含量最高，为33.24万SHU，小黄椒、A56和A61的辣椒素类物质含量在10~20万SHU，30份种质的辣椒素类物质含量均 < 10万SHU；在干物质辣椒红素含量方面，35份辣椒种质中含量 > 200 mg/kg的有6份，分别为遵辣1号、四平头、门巴辣椒、A59、遵义4号和A47，辣椒红素含量100~200 mg/kg的辣椒种质有8份，其他21份辣椒种质资源辣椒红素含量均 < 100 mg/kg；辣椒素类物质与辣椒红素含量之间的相关性不显著。研究揭示了不同辣椒种质在辣椒素类物质和辣椒红素含量上的显著差异，为辣椒育种提供了丰富的遗传资源，对选育高辣高红色素含量的品种奠定基础。

Abstract: In order to screen out excellent pepper germplasms with high capsaicinoids and high capsanthin content, 35 pepper germplasm resources as experimental materials were evaluated, and the contents of capsaicinoids and capsanthin were detected by high-performance liquid chromatography (HPLC) technology in the study. The detection method showed a good linear relationship, ensuring the accurate and reliable analytical results. Among the 35 pepper germplasms, Rela 6 had the highest capsaicinoids content, reaching 332,400 SHU. The capsaicinoids contents of Xiaohuangjiao, A56 and A61 ranged from 100,000 to 200,000 SHU, while the capsaicinoids content of 30 germplasms were all less than 100,000 SHU. In terms of the capsanthin content in dry matter, six of the 35 germplasms had capsanthin content exceeding 200 mg/kg, namely Zunla 1, Sipingtou, Menba Pepper, A59, Zunyi 4, and A47. Eight germplasms had capsanthin content ranging from 100 to 200 mg/kg, while the remaining 21 germplasms had capsanthin content less than 100 mg/kg. The correlation between capsaicinoids and capsanthin content was not significant. The study revealed significant differences in the contents of capsaicinoids and capsanthin among different pepper germplasms, providing rich genetic resources for pepper breeding and laying the foundation for the development of varieties with high capsaicinoids and high capsanthin content.