基于网络药理学探讨当归芍药散治疗黄褐斑的作用机制

Exploring the Mechanism of Danggui Shaoyao Powder in Treating Chloasma Based on Network Pharmacology

摘要: 目的：基于网络药理学方法研究当归芍药散治疗黄褐斑的作用机制。方法：使用TCMSP数据库、Gene Cards数据库、OMIM数据库、TTD数据库、Swiss Target Prediction数据库检索、筛选得出当归芍药散的有效活性成分、靶点及黄褐斑相关的疾病基因靶点，将所得数据导入VENNY2.1.0平台取得药物与疾病的交集靶点并绘制韦恩图。将两者间的交集数据输入STRING数据库构建蛋白互作网络图，并通过Cytoscape3.9.1软件构建复方–活性成分–靶点–药物网络。取靶点基因导入David数据库进行GO富集分析、KEGG富集分析。结果：取得当归芍药散有效活性成分26个，对应靶点1577个，主要活性成分包括山奈酚、芍药苷、类固醇类等；黄褐斑相关靶点785个；当归芍药散与黄褐斑交集靶点66个；GO分析共684个条目，包含508个生物过程条目、49个细胞组成条目、127个分子功能条目；KEGG分析共148个条目。且富集分析显示涉及通路包括生长因子信号通路、癌症通路、PI3K-AKT信号通路等等。结论：基于网络药理学研究预测当归芍药散治疗黄褐斑通过多活性成分–多靶点–多通路的综合调控网络发挥作用。

Abstract: Objective: To study the mechanism of Danggui Shaoyao Powder in the treatment of metasma based on network pharmacology. Methods: TCMSP database, GeneCards database, OMIM database, ITD database and Swiss-Target were used the active ingredients, targets and melasma-related disease gene targets of Danggui Shaoyaosan were obtained through Prediction database retrieval and screening, and the data were imported into VENNY2.1.0 platform to obtain the intersection targets of drugs and diseases and draw Wayne map. The intersection targets were selected to construct the protein interaction network through STRING database and Cytoscape3.9.1software, and the core targets were screened out to construct the compound, active ingredient-target-drug network. GO enrichment analysis and KEGG enrichment analysis were performed for target genes using David database. Results: 26 active components of Danggui Shaoyaosan were obtained, corresponding to 1577 targets. The main active components included kauniol, paeoniflorin, steroids, etc. There were 785 targets related to melasma; 66 intersection targets of Danggui Shaoyao SAN and melasma; A total of 684 items were analyzed by G0, including 508 items of biological process, 49 items of cell composition and 127 items of molecular function. A total of 148 items were analyzed by KEGG. Enrichment analysis showed that the pathways involved included growth factor signaling pathway, cancer signaling pathway, PI3K-AKT signaling pathway, and so on. Conclusion: Based on the study of network pharmacology, it is predicted that Danggui Shaoyao Powder plays a role in the treatment of melasma through the comprehensive regulatory network of multi-active ingredients, multi-target and multi-pathway.