纤维素酶协同提取鱼腥草多酚工艺优化及抑菌活性测定

Optimization of Cellulase Assisted Extraction of Polyphenols from Houttuynia cordata and Determination of Antibacterial Activity

摘要: 采用乙醇回流法从纤维素酶协同提取鱼腥草中多酚类化合物，通过正交试验对提取条件进行优化，结果显示，最佳提取条件为温度80℃、提取时间90 min、乙醇浓度40%、液料比1:60，多酚得率为2.16%；同时对粗提物抑制金黄色葡萄球菌金葡亚种的活性进行测定，结果表明该提取物对金黄色葡萄球菌金葡亚种具有明显抑菌效果，最小抑菌浓度为0.0187 (g/mL)、最小杀菌浓度为0.25 (g/mL)。

Abstract: The ethanol reflux method was used to synergistically extract polyphenolic compounds from Houttuynia cordata using cellulase. The extraction conditions were optimized through orthogonal experiments, and the results showed that the optimal extraction conditions were temperature of 80˚C, extraction time of 90 minutes, ethanol concentration of 40%, liquid to material ratio of 1:60, and polyphenol yield of 2.16%; At the same time, the activity of crude extract in inhibiting Staphylococcus aureus subsp. aureus was determined, and the results showed that the extract had a significant antibacterial effect on Staphylococcus aureus subsp. aureus, with a minimum inhibitory concentration of 0.0187 (g/mL) and a minimum bactericidal concentration of 0.25 (g/mL).