IL-6借助细胞焦亡刺激主动脉夹层的进展：一项基于已有数据库的差异性分析

IL-6 Stimulates Progression of Aortic Dissection with Pyroptosis: A Differential Analysis Based on Existing Databases

摘要: 目的：细胞焦亡是导致主动脉夹层的发生的重要的病理过程。本研究旨在在已有研究的基础上，进一步筛选鉴定与主动脉夹层相关的细胞焦亡的潜在生物学靶点。材料方法：首先，从基因表达综合数据库中筛选mRNA表达谱数据集GSE153434，并使用R软件(4.0.0版)分析与主动脉夹层和细胞焦亡相关的潜在差异表达基因。随后，对选定的候选基因进行蛋白质–蛋白质相互作用分析、基因本体富集分析和京都基因与基因组百科全书通路富集分析等。并最终根据hub基因的排名，筛选出可能的潜在靶向基因。结果：我们通过差异性分析确定了2369个可能与主动脉夹层相关的潜在靶点。通过结合开放的细胞焦亡数据库，我们进一步确认73个细胞焦亡相关的差异基因。蛋白质–蛋白质相互作用网络展示出绝大多数候选靶点存在密切的相互作用。最终我们再次从73个候选的潜在靶点基因中根据蛋白质–蛋白质相互作用网络中的评分，筛选了得分最高的作为关键基因：IL-6。结论：我们的研究鉴定了在主动脉夹层中73个潜在的细胞焦亡相关的差异表达基因。IL-6则可能作为关键枢纽基因参与主动脉夹层的发生。本研究结果可能有助于进一步了解主动脉夹层的发病机制，并指导治疗。

Abstract: Introduction: Pyroptosis is an important pathological process leading to aortic dissection. The aim of this study was to further screen and identify potential biological targets for cell pyrodeath associated with aortic dissection on the basis of previous studies. Material Method: Firstly, the mRNA expression profile dataset GSE153434 was screened from the comprehensive gene expression database, and the potential differentially expressed genes associated with aortic dissection and pyroptosis were analyzed using R software (version 4.0.0). Subsequently, protein-protein interaction analysis, gene ontology enrichment analysis and Kyoto Encyclopedia of Genes and genomes pathway enrichment analysis were performed on the selected candidate genes. Finally, according to the ranking of hub genes, possible potential target genes were screened out. Result: We identified 2369 possible potential targets associated with aortic dissection through differential analysis. By combining the open pyroptosis database, we further identified 73 differential genes associated with pyroptosis. Protein-protein interaction networks show close interactions at most candidate targets. Finally, we again selected the key gene with the highest score from 73 candidate potential target genes according to the score in the protein-protein interaction network: IL-6. Conclusion: Our study identified 73 differentially expressed genes potentially associated with pyroptosis in aortic dissection. IL-6 may be a key gene involved in the occurrence of aortic dissection. The results of this study may contribute to further understanding of the pathogenesis of aortic dissection and guide treatment.