运输胁迫对梭鲈应急指标的影响

Effect of Transport Stress on Physicochemical Parameters of Sander lucioperca

摘要: 该实验旨在研究梭鲈在运输胁迫后肌肉、内脏团、鳃理化指标含量变化。选取200尾平均体重为(2.67 ± 0.83) g的梭鲈为实验对象，本研究设置七个实验组，分别研究了在进行轿车2小时运输胁迫后，0 h，3 h，6 h，12 h，24 h，48 h，96 h，梭鲈理化指标的变化。结果表明，在短途运输胁迫下，运输胁迫对内脏团MDA含量的影响总体的趋势是呈下降后上升，对内脏团SOD活性、内脏团T-AOC、内脏团CAT活力的影响表现为先上升后下降再上升后下降，对内脏团GSH含量的影响呈下降后上升再下降，之后上升最后下降，运输胁迫前GSH含量最高，对内脏团GSH-PX酶活力，在运输胁迫后48 h开始上升，对肌肉LZM (溶菌酶)含量总体趋势为先上升后下降，对内脏团AKP含量、肌肉LDH活力的影响趋势总体呈上升后下降再上升，对鳃NA+-K+ ATP酶活力的影响总体趋势为下降后上升，下降后恢复至运输胁迫前水平。结果表明，短途运输胁迫对梭鲈造成的应激反应，在96 h后部分内脏团理化指标趋于胁迫前水平，对其生理机能不会产生持久性的影响。

Abstract: The experiment aims to study the changes in the content of muscle, visceral mass and gill physical and chemical indexes of spindle perch after transport stress. The 200-tailed shuttle bass with an average weight of (2.67 ± 0.83) g was selected as the experimental object. Seven experimental groups were set up in this study to study the changes of the physical and chemical indicators of 0 h, 3 h, 6 h, 12 h, 24 h, 48 h, 96 h, and shuttle bass after 2 hours of transportation stress. The results show that under short-distance transportation stress, the overall trend of the impact of transport stress on the MDA content of the visceral group is to rise after decline, the impact on the SOD activity of the visceral group T-AOC, and the CAT vitality of the viscera group is to rise first rise and then fall, then rise and then fall, the impact on the GSH content of the of the visceral group decreases and then rises and then decreases, and finally declines. The GSH content before the transport stress is the highest. The GSH-PX enzyme activity of the viscera group begins to rise 48 hours after the transport stress, and the overall trend of the content of muscle LZM (lysosozyme) is to rise first and then decreases. The overall trend of the influence on the AKP content and muscle LDH activity of the viscer The results show that the stress response caused by short-distance transport stress on shuttle perch tends to be at the pre-stress level after 96 hours, and will not have a lasting impact on its physiological function.