PEG联合血府逐瘀汤治疗小鼠脊髓损伤的疗效研究
Efficacy Study of PEG Combined with Xuefu Zhuyu Decoction in Treating Spinal Cord Injury in Mice
DOI: 10.12677/tcm.2025.146379, PDF,    科研立项经费支持
作者: 王智慧*：广西中医药大学研究生院，广西 南宁；任晓平#：广西中医药大学研究生院，广西 南宁；广西中医药大学附属瑞康医院修复重建显微外科，广西 南宁
关键词: 脊髓损伤；聚乙二醇；血府逐瘀汤；轴突；胶质瘢痕；Spinal Cord Injury； Polyethylene Glycol； Xuefu Zhuyu Decoction； Axon； Glial Scar
摘要: 目的：探索聚乙二醇(polyethylene glycol, PEG)联合血府逐瘀汤治疗急性脊髓损伤(spinal cord injury, SCI)的疗效及作用机制，优化PEG介导脊髓轴突的融合与再生的核心技术，为临床治疗提供新思路。方法：建立雌性C57BL/6J小鼠急性完全性脊髓横断模型，随机分为假手术组(sham组)、SCI模型组(SCI组)、PEG治疗组(SCI + PEG组)及PEG联合血府逐瘀汤组(SCI + PEG + 血府逐瘀汤组)。术后21天，通过Basso小鼠运动量表(BMS)评估后肢运动功能。结果：与sham组相比，SCI组、SCI + PEG组、SCI + PEG + 血府逐瘀汤组分别在术后第1天、第3天、第7天、第14天、第21天的小鼠后肢BMS评分显著降低(均P < 0.05)；与SCI组相比，SCI + PEG组及SCI + PEG + 血府逐瘀汤组在术后第7天、第14天、第21天的BMS评分显著增高(均P < 0.05)。结论：1. PEG通过融合轴突断端、恢复电信号传导，显著改善SCI后运动功能状态。2. 血府逐瘀汤通过改善微循环、抑制氧化应激，与PEG协同促进轴突再生、髓鞘修复及瘢痕抑制。3. PEG联合血府逐瘀汤为SCI修复提供了更多的干预策略，具有潜在临床应用价值。
Abstract: Objective: To explore the efficacy and mechanism of polyethylene glycol (PEG) combined with Xuefu Zhuyu Decoction in treating acute spinal cord injury (SCI), and to optimize the core technology of PEG-mediated axonal fusion and regeneration in spinal cord repair, providing new ideas for clinical treatment. Methods: An acute complete spinal cord transection model was established in female C57BL/6J mice. The mice were randomly divided into the sham group, SCI model group, PEG treatment group (SCI + PEG), and PEG combined with Xuefu Zhuyu Decoction group (SCI + PEG + Xuefu Zhuyu Decoction). The Basso Mouse Scale (BMS) was used to assess hindlimb motor function on day 1, 3, 7, 14, and 21 post-surgery. Results: Compared with the sham group, the BMS scores of the SCI group, SCI + PEG group, and SCI + PEG + Xuefu Zhuyu Decoction group were significantly lower on day 1, 3, 7, 14, and 21 post-surgery (P < 0.05); Compared with the SCI group, the BMS scores of the SCI + PEG group and SCI + PEG + Xuefu Zhuyu Decoction group were significantly higher on day 7, 14, and 21 post-surgery(P < 0.05). Conclusion: 1. PEG significantly improves motor function after SCI by fusing the axonal ends and restoring electrical signal conduction. 2. Xuefu Zhuyu Decoction improves microcirculation, inhibits oxidative stress, and synergistically promotes axonal regeneration, myelin repair, and scar suppression when combined with PEG. 3. The combination of PEG and Xuefu Zhuyu Decoction provides additional intervention strategies for SCI repair and has potential clinical application value.
文章引用：王智慧, 任晓平. PEG联合血府逐瘀汤治疗小鼠脊髓损伤的疗效研究[J]. 中医学, 2025, 14(6): 2568-2576. https://doi.org/10.12677/tcm.2025.146379

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