三七与尼莫地平治疗脑梗死的代谢组学机制及协同作用研究进展

Metabolomics Mechanisms and Synergistic Effects of Panax Notoginseng and Nimodipine in Treating Cerebral Infarction: A Research Progress

摘要: 本研究基于代谢组学技术，探讨三七总皂苷(PNS)与尼莫地平单用及联用干预脑梗死大鼠模型的代谢调控机制及协同效应。结果表明：PNS通过调节能量代谢(如三羧酸循环)、氨基酸代谢(如谷氨酸平衡)、脂质代谢及抗氧化途径(提升谷胱甘肽水平)，改善脑缺血损伤；尼莫地平则主要调控钙离子信号通路、能量代谢(AMPK通路)及细胞凋亡相关代谢(抑制caspase-3)，减轻神经损伤。二者联用可协同优化能量代谢、减轻氧化应激(降低MDA、提升SOD)及炎症反应，促进神经功能恢复，效果显著优于单药治疗。研究筛选出12种潜在代谢生物标志物(如牛磺酸、乳酸)，为临床联合用药提供理论依据。

Abstract: This study employed metabolomics technology to investigate the metabolic regulatory mechanisms and synergistic effects of Panax notoginseng saponins (PNS) and nimodipine, both individually and in combination, in a rat model of cerebral infarction. The results demonstrated that PNS ameliorated cerebral ischemic injury by regulating energy metabolism (e.g., the tricarboxylic acid (TCA) cycle), amino acid metabolism (e.g., glutamate balance), lipid metabolism, and antioxidant pathways (e.g., increasing glutathione levels). Nimodipine primarily alleviated neuronal damage by modulating calcium ion signaling pathways, energy metabolism (AMPK pathway), and apoptosis-related metabolism (e.g., inhibiting caspase-3). The combination therapy synergistically optimized energy metabolism, alleviated oxidative stress (reducing malondialdehyde (MDA) and increasing superoxide dismutase (SOD)) and inflammation, and promoted neurological functional recovery, with effects significantly superior to monotherapy. The study identified 12 potential metabolic biomarkers (e.g., taurine, lactate), providing a theoretical basis for the clinical application of the combination therapy.