基于数据挖掘分析淫羊藿抗缺血性脑卒中作用机制研究
Analysis of Mechanism of Epimedium against Ischemic Stroke Based on Data Mining
DOI: 10.12677/tcm.2025.148534, PDF,    科研立项经费支持
作者: 笪小云*：贵州中医药大学基础医学院，贵州 贵阳；固镇县中医院内科，安徽 蚌埠；刘 明#：贵州中医药大学基础医学院，贵州 贵阳；贵州中医药大学中药、民族药药理作用及作用机制研究中心，贵州 贵阳
关键词: 淫羊藿；缺血性脑卒中；作用机制；网络药理学；Epimedium； Ischemic Stroke； Mechanism of Action； Network Pharmacology
摘要: 目的：基于数据挖掘探究淫羊藿抗缺血性脑卒中的作用机制。方法：六大数据库分别搜集药物成分及靶点、疾病靶蛋白；并建立蛋白质相互作用网络(即PPI网络)：GO功能、KEGG通路富集分析；最后分子对接可视化。结果：淫羊藿与缺血性脑卒中交集基因125个，蛋白互作分析得到核心作用靶点为TP53(66)、IL6 (42)、MAPK1 (34)、CASP3 (36)、TNF (44)、ESR1 (34)、BCL2 (36)、AKT1 (56)，GO富集分析涵盖生物功能(对脂多糖的反应等)、细胞组分(囊腔等)、分子功能(DNA结合转录因子结合等)。KEGG分析得出AGE-RAGE等信号通路为其作用主要信号通路。结论：淫羊藿通过多途径、多靶点作用于缺血性脑卒中。
Abstract: Objective: To explore the mechanism of anti-ischemic stroke in Epimedium based on data mining. Methods: Chemical constituents and corresponding targets of Herba Epimedium were collected from TCM database. Target proteins of “Ischemic Stroke” were collected from five major databases: protein-protein interaction network established in STRING database. GO functional enrichment and KEGG pathway enrichment analysis: results obtained by autodock vina molecular docking visualization. Results: 125 genes overlapped with ischemic stroke in Epimedium. The core targets of protein interaction were TP53 (66), IL6 (42), MAPK1 (34), CASP3 (36), TNF (44), ESR1 (34), BCL2 (36), AKTI (56). GO enrichment analysis covered biological functions (response to lipopolysaccharides, etc.), cellular components (sac lumen, etc.), and molecular functions (DNA-binding transcription factor binding, etc.). KEGG analysis showed that AGE-RAGE and other signaling pathways were the main signaling pathways. Conclusion: Epimedium plays a role in the treatment of ischemic stroke through multiple pathways and multiple targets.
文章引用：笪小云, 刘明. 基于数据挖掘分析淫羊藿抗缺血性脑卒中作用机制研究[J]. 中医学, 2025, 14(8): 3653-3662. https://doi.org/10.12677/tcm.2025.148534

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