基于分子内电荷转移机理的锌离子荧光探针L1的检测机制研究
Research on the Detection Mechanism of Zinc Ion Fluorescent Probe L1 Based on the Intramolecular Charge Transfer Mechanism
DOI: 10.12677/ojns.2025.135094, PDF,    科研立项经费支持
作者: 徐嘉彤, 彭永进, 刘玉玲*, 张右梓*：锦州医科大学健康管理现代产业学院，辽宁 锦州；庞楚璇：锦州医科大学附属第一医院，辽宁 锦州
关键词: 比率型荧光探针；锌离子；分子内电荷转移；荧光检测；理论计算；Ratiometric Fluorescent Probe； Zinc Ion； Intramolecular Charge Transfer； Fluorescence Detection； Theoretical Calculation
摘要: 本文详细研究了基于分子内电荷转移(Intramolecular Charge Transfer, ICT)机理设计合成的比率型荧光探针L1用于检测溶液中锌离子的检测机制。通过对探针L1及其与锌离子络合物L1-Zn的光谱实验结果的理论计算和分析，深入探讨了探针L1对锌离子的识别检测机制，为新型高效金属离子荧光探针的设计提供了理论依据和新思路。
Abstract: This paper conducts an in-depth study on the detection mechanism of ratiometric fluorescent probe L1, which is designed and synthesized based on the intramolecular charge transfer (ICT) mechanism, for detecting zinc ions in solution. Through theoretical calculations and investigation on spectroscopic experiments of probe L1 and its complex L1-Zn with zinc ions, the recognition and detection mechanism of probe L1 for zinc ions is explored in detail. This study provides a theoretical basis and novel design ideas for the development of new, efficient fluorescent probes for metal ions.
文章引用：徐嘉彤, 彭永进, 庞楚璇, 刘玉玲, 张右梓. 基于分子内电荷转移机理的锌离子荧光探针L1的检测机制研究[J]. 自然科学, 2025, 13(5): 897-904. https://doi.org/10.12677/ojns.2025.135094

参考文献

[1] You, B., Li, L., Li, Z., Wang, W., Yang, Y., Cheng, W., et al. (2025) Imaging of Zinc Ions across Diverse Biological Samples with a Quinoline-Based Tris(2-Pyridylmethyl)Amine Fluorescent Probe. Talanta, 284, Article ID: 127267. [Google Scholar] [CrossRef] [PubMed]
[2] Kim, H., Cho, E., Kwak, M., Lee, J., Lee, H., Hwang, C., et al. (2025) Porphyrinic N4 Channels of Zinc Ions for the Electrochemical Reversibility of Zinc Plating/stripping. Materials Horizons, 12, 1651-1662. [Google Scholar] [CrossRef] [PubMed]
[3] Alam, M.Z., Ahmad, S., Alimuddin, and Khan, S.A. (2024) Synthesis of Fluorescent Pyrazoline Sensors as Versatile Tool for Zinc Ion Detection: A Mini-Review. Journal of Fluorescence, 35, 1241-1253. [Google Scholar] [CrossRef] [PubMed]
[4] Maret, W. (2024) Chemistry Meets Biology in the Coordination Dynamics of Metalloproteins. Journal of Inorganic Biochemistry, 251, Article ID: 112431. [Google Scholar] [CrossRef] [PubMed]
[5] Guo, Z., Liu, Z., Wang, P., Zhao, C., Lu, X., Zhang, Y., et al. (2024) Biomineralization Inspired the Construction of Dense Spherical Stacks for Dendrite-Free Zinc Anodes. Nano Letters, 24, 14656-14662. [Google Scholar] [CrossRef] [PubMed]
[6] Wang, Y., Huang, N. and Yang, Z. (2023) Revealing the Role of Zinc Ions in Atherosclerosis Therapy via an Engineered Three‐Dimensional Pathological Model. Advanced Science, 10, Article ID: 2300475. [Google Scholar] [CrossRef] [PubMed]
[7] Yoon, C. and Lee, S.J. (2021) Selective Coordination of Cobalt Ions by Zinc Fingers in Escherichia coli. Bulletin of the Korean Chemical Society, 42, 1650-1658. [Google Scholar] [CrossRef] 
[8] Xu, H., Luo, Y., Tu, X., Cui, W., Dou, Y. and Wang, Q. (2021) Effect of the Forth and Fifth Zinc Finger Deletions of MTF-1 on the Expression of Metal Ion Metabolism Related Gene. Doklady Biochemistry and Biophysics, 500, 385-392. [Google Scholar] [CrossRef] [PubMed]
[9] Missirlis, F. (2021) Regulation and Biological Function of Metal Ions in Drosophila. Current Opinion in Insect Science, 47, 18-24. [Google Scholar] [CrossRef] [PubMed]
[10] Nathani, S., Kumar, V., Dhaliwal, H.S., Sircar, D. and Roy, P. (2020) Biological Application of a Fluorescent Zinc Sensing Probe for the Analysis of Zinc Bioavailability Using Caco-2 Cells as an In-Vitro Cellular Model. Journal of Fluorescence, 30, 1553-1565. [Google Scholar] [CrossRef] [PubMed]
[11] Chen, S., Sun, T., Xie, Z., Dong, D. and Zhang, N. (2020) A Fluorescent Sensor for Intracellular Zn2+ Based on Cylindrical Molecular Brushes of Poly(2-Oxazoline) through Ion-Induced Emission. Polymer Chemistry, 11, 6650-6657. [Google Scholar] [CrossRef] 
[12] Rogina, A., Loncarevic, A., Antunovic, M., Marijanovic, I., Ivankovic, M. and Ivankovic, H. (2019) Tuning Physicochemical and Biological Properties of Chitosan through Complexation with Transition Metal Ions. International Journal of Biological Macromolecules, 129, 645.
[13] Jonaghani, M.Z., Zali-Boeini, H. and Moradi, H. (2019) A Coumarin Based Highly Sensitive Fluorescent Chemosensor for Selective Detection of Zinc Ion. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 207, 16-22. [Google Scholar] [CrossRef] [PubMed]
[14] Zhao, D., Zhang, W., Zhang, S. and Shen, G. (2025) Recent Advances in Lithium Ion Sensing Using Fluorescent Probes with Different Structures. ChemistrySelect, 10, e01543. [Google Scholar] [CrossRef] 
[15] Shen, J., Wang, X., Qin, L., Sun, L., Liang, Y., Li, R., et al. (2025) Ratiometric Fluorescent Probes Based on Fluorogenic Reactions of O-Phenylenediamine for Multiple Sensing Applications. Talanta, 295, Article ID: 128329. [Google Scholar] [CrossRef] [PubMed]
[16] Jiang, X., Yang, R., Lei, X., Xue, S., Wang, Z., Zhang, J., et al. (2023) Design, Synthesis, Application and Research Progress of Fluorescent Probes. Journal of Fluorescence, 34, 965-975. [Google Scholar] [CrossRef] [PubMed]
[17] Huang, Y., Cao, X., Deng, Y., Ji, X., Sun, W., Xia, S., et al. (2024) An Overview on Recent Advances of Reversible Fluorescent Probes and Their Biological Applications. Talanta, 268, Article ID: 125275. [Google Scholar] [CrossRef] [PubMed]
[18] Yang, J., Zhao, Z., Jiang, S., Zhang, L., Zhao, K., Li, Z., et al. (2023) Ph-sensing Supramolecular Fluorescent Probes Discovered by Library Screening. Talanta, 263, Article ID: 124716. [Google Scholar] [CrossRef] [PubMed]
[19] Yan, L., Yang, H., Zhang, S., Zhou, C. and Lei, C. (2022) A Critical Review on Organic Small Fluorescent Probes for Monitoring Carbon Monoxide in Biology. Critical Reviews in Analytical Chemistry, 53, 1792-1806. [Google Scholar] [CrossRef] [PubMed]
[20] Xue, X., Wang, Y., Chen, S., Wang, K., Niu, S., Zong, Q., et al. (2023) Monitoring Intracellular pH Using a Hemicyanine-Based Ratiometric Fluorescent Probe. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 284, Article ID: 121778. [Google Scholar] [CrossRef] [PubMed]
[21] Lee, H., Lee, S. and Han, M.S. (2023) Turn-On Fluorescent pH Probes for Monitoring Alkaline pHs Using Bis[2-(2’-Hydroxyphenyl)Benzazole] Derivatives. Sensors, 23, Article 2044. [Google Scholar] [CrossRef] [PubMed]
[22] Fu, L., Huang, H., Zuo, Z. and Peng, Y. (2023) A Single Organic Fluorescent Probe for the Discrimination of Dual Spontaneous ROS in Living Organisms: Theoretical Approach. Molecules, 28, Article 6983. [Google Scholar] [CrossRef] [PubMed]
[23] Yan, A., Wang, C., Yan, J., Wang, Z., Zhang, E., Dong, Y., et al. (2023) Thin‐Film Transistors for Integrated Circuits: Fundamentals and Recent Progress. Advanced Functional Materials, 34, Article ID: 2304409. [Google Scholar] [CrossRef] 
[24] Nakata, E., Gerelbaatar, K., Komatsubara, F. and Morii, T. (2022) Stimuli-responsible SNARF Derivatives as a Latent Ratiometric Fluorescent Probe. Molecules, 27, Article 7181. [Google Scholar] [CrossRef] [PubMed]
[25] Du, K., Niu, S., Qiao, L., Dou, Y., Zhu, Q., Chen, X., et al. (2017) A Highly Selective Ratiometric Fluorescent Probe for the Cascade Detection of Zn2+ and H2po4− and Its Application in Living Cell Imaging. RSC Advances, 7, 40615-40620. [Google Scholar] [CrossRef] 
[26] Huang, H., Zou, Z. and Peng, Y. (2024) Theoretical Insights into a Turn-On Fluorescence Probe Based on Naphthalimide for Peroxynitrite Detection. Heliyon, 10, e37298. [Google Scholar] [CrossRef] [PubMed]
[27] Deng, Y., Huang, H., Feng, J., Peng, Y. and Liu, Y. (2024) Theoretical Investigation of a Coumarin Fluorescent Probe for Distinguishing the Detection of Small-Molecule Biothiols. Molecules, 29, Article 554. [Google Scholar] [CrossRef] [PubMed]
[28] Peng, Y., Huang, H., Liu, Y. and Zhao, X. (2023) Theoretical Insights into a Near-Infrared Fluorescent Probe NI-VIS Based on the Organic Molecule for Monitoring Intracellular Viscosity. Molecules, 28, Article 6105. [Google Scholar] [CrossRef] [PubMed]
[29] Lu, T. (2024) A Comprehensive Electron Wavefunction Analysis Toolbox for Chemists, Multiwfn. The Journal of Chemical Physics, 161, Article ID: 082503. [Google Scholar] [CrossRef] [PubMed]
[30] Humphrey, W., Dalke, A. and Schulten, K. (1996) VMD: Visual Molecular Dynamics. Journal of Molecular Graphics, 14, 33-38. [Google Scholar] [CrossRef] [PubMed]