RT-PCR快速检测技术用于食品金黄色葡萄球菌检测的价值研究
Research on the Value of RT-PCR Rapid Detection Technology in the Detection of Staphylococcus aureus in Food
摘要: 探讨RT-PCR快速检测技术在食品金黄色葡萄球菌检测中的准确性、灵敏度、特异性，以此为基础分析该技术的实际应用价值。采集各类食品样本用于对比实验，设置对照组和观察组。对照组利用常规的微生物培养法进行检测，观察组则使用RT-PCR快速检测技术进行检测。对比研究2组样本在阳性检出率、检测时间、灵敏度、特异度等方面的差异。
Abstract: This paper explores the accuracy, sensitivity and specificity of RT-PCR rapid detection technology in the detection of Staphylococcus aureus in food, and analyzes the practical application value of this technology based on this. Various food samples were collected for comparative experiments, and a control group and an observation group were set up. The control group was detected by conventional microbial culture methods, while the observation group was detected by RT-PCR rapid detection technology. A comparative study was conducted on the differences between the two groups of samples in terms of positive detection rate, detection time, sensitivity and specificity.
参考文献
|
[1]
|
吴仔东. 食品中金黄色葡萄球菌快速检测方法的研究进展[J]. 中外食品工业, 2024(17): 31-33.
|
|
[2]
|
付燕峰, 娄亚坤, 苗银萍, 等. 恒温荧光扩增法快速检测食品中金黄色葡萄球菌[J]. 实验室检测, 2023, 1(3): 21-29.
|
|
[3]
|
谭慧林, 吴忠红, 金永生, 等. 食品中金黄色葡萄球菌的快速检测及其评估[J]. 新疆农业科学, 2022, 59(2): 410-416.
|
|
[4]
|
李寅生, 王璐, 张懿雪, 等. BAXQ7系统快速检测食品中金黄色葡萄球菌效果验证[J]. 食品安全质量检测学报, 2020, 11(10): 3256-3260.
|
|
[5]
|
张小苗. 基因探针法应用于食品中金黄色葡萄球菌快速检测的效果[J]. 生物技术世界, 2015(9): 12.
|
|
[6]
|
胡守奎, 胡万富, 撒楠. 应用测试片法快速检测食品中的金黄色葡萄球菌[J]. 安徽预防医学杂志, 2011, 17(5): 393-394.
|
|
[7]
|
李荔枝, 胡萍. 快速检测食品中金黄色葡萄球菌及其肠毒素型的研究进展[J]. 江西农业学报, 2011, 23(8): 144-146.
|
|
[8]
|
徐义刚, 李苏龙, 李丹丹, 等. 食品中金黄色葡萄球菌DNA环介导恒温扩增快速检测方法的建立与应用[J]. 中国农业科学, 2010, 43(8): 1655-1663.
|
|
[9]
|
黄岭芳, 赖卫华, 张莉莉. 食品中金黄色葡萄球菌快速检测方法的研究进展[J]. 食品与机械, 2009, 25(6): 181-185.
|
|
[10]
|
许如苏, 林彩华, 孙霞, 等. 应用测试片快速检测食品中金黄色葡萄球菌的研究[J]. 食品研究与开发, 2009, 30(1): 94-97.
|