基于网络药理学探讨千金苇茎汤“异病同治”支气管扩张症和哮喘急性发作的作用机制

Exploring the Mechanism of Qianjin Weigan Tang’s “Treating Different Diseases with the Same Treatment” for Bronchiectasis and Acute Asthma Attacks Based on Network Pharmacology

摘要: 目的：基于网络药理学探讨千金苇茎汤“异病同治”支气管扩张症和哮喘急性发作的作用机制。方法：检索TCMSP数据库获取千金苇茎汤中中药活性成分，预测其作用靶点，利用UniProt数据库转换靶点基因信息；应用GeneCards、OMIM和DrugBank数据库筛选两种疾病相关疾病靶点；利用微生信平台绘制韦恩图，将获得的靶点蛋白与疾病靶点进行汇总取交集，获得三者共有靶点；将交集靶点导入String数据库构建核心靶点蛋白质相互作用图，通过Cytoscape 3.7.2构建“药物活性成分–靶点–疾病”网络图；根据度值筛选千金苇茎汤治疗支气管扩张症和哮喘急性发作的关键靶点基因，利用David数据库进行GO功能和KEGG通路富集分析，使用微生信平台进行可视化处理。结果：筛选后得到千金苇茎汤有效成分35个，作用靶点176个，与两种疾病三者交集靶点9个；“药物活性成分–靶点–疾病”网络图获豆甾醇、β-谷甾醇、常春藤皂苷元等11个主要活性成分；PPI网络图中核心靶点为AKT1、EGFR、HSP90AA1等7个；GO功能富集分析涉及细胞组成(CC) 20项，分子功能(MF) 22项，生物过程(BP) 61项；KEGG通路富集分析主要涉及ErbB信号通路、PI3K-Akt信号通路、血小板活化、磷脂酶D信号通路等通路。结论：千金苇茎汤通过多种药物成分，调节多条信号通路，关联多个关键靶点，从而发挥对支气管扩张症和哮喘急性发作的“异病同治”作用。

Abstract: Objective: To explore the mechanism of action of Qianjin Weigan Tang in treating bronchiectasis and acute asthma attacks with “treating different diseases with the same treatment” based on network pharmacology. Method: Search the TCMSP database to obtain the active ingredients of traditional Chinese medicine in Qianjin Weigan Tang, predict their target of action, and use the UniProt database to convert target gene information; Screening two disease-related disease targets using GeneCards, OMIM, and DrugBank databases; Using the WeChat platform to draw a Venn diagram, the obtained target proteins are summarized and intersected with disease targets to obtain common targets among the three; Import the intersection targets into the String database to construct a core target protein interaction diagram, and use Cytoscape 3.7.2 to construct a “drug active ingredient target disease” network diagram; Based on the degree value, key target genes for the treatment of bronchiectasis and acute asthma attacks with Qianjin Weigan decoction were screened. GO function and KEGG pathway enrichment analysis were performed using the David database, and visualized using a bioinformatics platform. Result: After screening, 35 active ingredients and 176 targets of Qianjin Weigan Tang were obtained, including 9 intersecting targets with two diseases; The network diagram of “active ingredients target disease” obtained 11 main active ingredients including stigmasterol, β-sitosterol, and hederagenin; The core targets in the PPI network diagram are AKT1, EGFR, HSP90AA1, and 7 others; GO functional enrichment analysis involves 20 cellular components (CC), 22 molecular functions (MF), and 61 biological processes (BP); KEGG pathway enrichment analysis mainly involves pathways such as ErbB signaling pathway, PI3K Akt signaling pathway, platelet activation, phospholipase D signaling pathway, etc. Conclusion: Qianjin Weigan Tang exerts a “same treatment for different diseases” effect on bronchiectasis and acute asthma attacks by regulating multiple signaling pathways and linking multiple key targets through multiple drug components.