微针辅助快速DNA提取联合LAMP技术用于三文鱼的鉴别

Microneedle-Assisted Rapid DNA Extraction Coupled with LAMP for Salmon Authentication

摘要: 目的：开发一种基于微针贴片提取DNA并联合LAMP技术识别特定DNA的方法，实现特殊样本的快速采集与鉴别，为市场监管、企业自检提供便携化解决方案。方法：构建一种微针贴片以快速提取组织间液并采用吹打的方式洗脱DNA，利用可视化LAMP比色法实现三文鱼的DNA鉴别。结果：我们构筑的微针具有良好的外观表型、穿刺能力、抗形变能力与汲取能力，能够辅助快速DNA提取联合可视化LAMP比色法，可以在1 h内完成三文鱼的鉴别。结论：本方法构建的微针能够实现DNA快速提取，联合可视化LAMP比色法可快速实现对特定食品的鉴别，为食品的市场监管提供科学依据。

Abstract: Objective: To develop a microneedle patch that could rapidly extract DNA and combine it with loop-mediated isothermal amplification (LAMP) for the on-site identification of specific DNA, which enabled rapid collection and authentication of challenging samples and providing a portable tool for market surveillance and in-house quality control. Methods: A microneedle patch was constructed to collect interstitial fluid within minutes. The DNA was eluted by simple pipetting and immediately subjected to a visual LAMP assay designed to discriminate salmon DNA via colorimetric readout. Results: The optimized microneedle patch exhibited excellent structural integrity, high mechanical robustness, and efficient fluid uptake capacity. Combined with the colorimetric LAMP assay, it enabled unambiguous salmon authentication within one hour. Conclusion: The microneedle constructed by this method can achieve rapid DNA extraction, and combined with the visual LAMP colorimetric method, it can quickly identify specific foods, providing a scientific basis for food market supervision.