响应面法优化深共晶溶剂提取槐米总黄酮的工艺研究
Optimization of Deep Eutectic Solvent Extraction Process for Total Flavonoids from Sophora japonica Buds Using Response Surface Methodology
DOI: 10.12677/ojns.2025.135111, PDF,    科研立项经费支持
作者: 邹书玲, 黄 慧, 陈丽娟, 李居华, 丁鹏鹏*：贵州工程应用技术学院化学工程学院，贵州 毕节
关键词: 响应面法；深共晶溶剂；槐米；总黄酮；Response Surface Methodology； Deep Eutectic Solvent； Sophora japonica Buds； Total Flavonoids
摘要: 本研究运用响应面法对超声辅助深共晶溶剂提取槐米总黄酮的工艺进行优化。选取氯化胆碱与1,2-丙二醇(摩尔比1:2，含水量10%)作为提取溶剂，在单因素试验基础上，将液料比、提取时间、提取温度确定为关键变量，以总黄酮提取含量为响应值，进行Box-Behnken设计，构建了二次多项式回归模型Y = 5.45 + 0.71A − 0.21B + 0.40C + 0.24AB − 0.84AC + 0.028BC − 1.40A2 − 1.00B2 + 0.74C2。当液料比为35 mL/g、提取时间为2.5 h、提取温度为48℃时，槐米总黄酮提取含量可达6.11 mg/g。研究结果显示，响应面法能够高效优化DES提取工艺，为槐米总黄酮的绿色提取提供技术支持。
Abstract: This study employed response surface methodology (RSM) to optimize the ultrasound-assisted deep eutectic solvent (DES) extraction process for total flavonoids from Sophora japonica Buds. A DES composed of choline chloride and 1,2-propanediol (molar ratio 1:2, with 10% water content) was selected as the extraction solvent. Based on single-factor experiments, the liquid-to-material ratio, extraction time, and extraction temperature were identified as key variables. Using the total flavonoid extraction yield as the response value, a Box-Behnken design was implemented, establishing the quadratic polynomial regression model: Y = 5.45 + 0.71A − 0.21B + 0.40C + 0.24AB − 0.84AC + 0.028BC − 1.40A2 − 1.00B2 + 0.74C2. The optimal conditions were determined as: liquid-to-material ratio 35 mL/g, extraction time 2.5 h, and extraction temperature 48˚C, achieving a maximum total flavonoid yield of 6.11 mg/g. The results demonstrate that RSM effectively optimizes the DES extraction process, providing technical support for the green extraction of total flavonoids from Sophora japonica Buds.
文章引用：邹书玲, 黄慧, 陈丽娟, 李居华, 丁鹏鹏. 响应面法优化深共晶溶剂提取槐米总黄酮的工艺研究[J]. 自然科学, 2025, 13(5): 1055-1065. https://doi.org/10.12677/ojns.2025.135111

参考文献

[1] Gong, Y., Fan, L., Wang, L. and Li, J. (2021) Flos Sophorae Immaturus: Phytochemistry, Bioactivities, and Its Potential Applications. Food Reviews International, 39, 3185-3203. [Google Scholar] [CrossRef] 
[2] Shi, P., Liao, J., Duan, T., Wu, Q., Huang, X., Pei, X., et al. (2023) Chemical Composition and Pharmacological Properties of Flos Sophorae Immaturus, Flos Sophorae and Fructus Sophorae: A Review. Journal of Future Foods, 3, 330-339. [Google Scholar] [CrossRef] 
[3] Zhao, X., Zou, X., Li, Q., Cai, X., Li, L., Wang, J., et al. (2018) Total Flavones of Fermentation Broth by Co-Culture of Coprinus comatus and Morchella esculenta Induces an Anti-Inflammatory Effect on Lps-Stimulated RAW264.7 Macrophages Cells via the MAPK Signaling Pathway. Microbial Pathogenesis, 125, 431-437. [Google Scholar] [CrossRef] [PubMed]
[4] Oke, O.E., Adeyi, O., Okolo, B., Adeyi, J., Nnabodo, D., Ude, C.J., et al. (2023) Solid-Liquid Microwave-Assisted Extraction of Bioactive Extract Recovery from Hunteria umbellata Seeds: Non-Mechanistic Modelling, Bi-Objective Optimization, HPLC Finger Printing and Scale-Up Techno-Economics with Sensitivity Analysis. Cleaner Chemical Engineering, 5, Article ID: 100097. [Google Scholar] [CrossRef] 
[5] Atwi-Ghaddar, S., Destandau, E. and Lesellier, E. (2023) Optimization of Supercritical Fluid Extraction of Polar Flavonoids from Robinia pseudoacacia L. Heartwood. Journal of CO2 Utilization, 70, Article ID: 102440. [Google Scholar] [CrossRef] 
[6] Hu, Q., Yu, J., Yang, W., Kimatu, B.M., Fang, Y., Ma, N., et al. (2016) Identification of Flavonoids from Flammulina velutipes and Its Neuroprotective Effect on Pheochromocytoma-12 Cells. Food Chemistry, 204, 274-282. [Google Scholar] [CrossRef] [PubMed]
[7] Poojary, M., Barba, F., Aliakbarian, B., Donsì, F., Pataro, G., Dias, D., et al. (2016) Innovative Alternative Technologies to Extract Carotenoids from Microalgae and Seaweeds. Marine Drugs, 14, Article No. 214. [Google Scholar] [CrossRef] [PubMed]
[8] Petrotos, K., Giavasis, I., Gerasopoulos, K., Mitsagga, C., Papaioannou, C. and Gkoutsidis, P. (2021) Optimization of Vacuum-Microwave-Assisted Extraction of Natural Polyphenols and Flavonoids from Raw Solid Waste of the Orange Juice Producing Industry at Industrial Scale. Molecules, 26, Article No. 246. [Google Scholar] [CrossRef] [PubMed]
[9] Krakowska-Sieprawska, A., Rafińska, K., Walczak-Skierska, J., Kiełbasa, A. and Buszewski, B. (2021) Promising Green Technology in Obtaining Functional Plant Preparations: Combined Enzyme-Assisted Supercritical Fluid Extraction of Flavonoids Isolation from Medicago Sativa Leaves. Materials, 14, Article No. 2724. [Google Scholar] [CrossRef] [PubMed]
[10] Wang, G., Cui, Q., Yin, L., Zheng, X., Gao, M., Meng, Y., et al. (2019) Efficient Extraction of Flavonoids from Flos Sophorae Immaturus by Tailored and Sustainable Deep Eutectic Solvent as Green Extraction Media. Journal of Pharmaceutical and Biomedical Analysis, 170, 285-294. [Google Scholar] [CrossRef] [PubMed]
[11] Peng, F., Xu, P., Zhao, B., Zong, M. and Lou, W. (2018) The Application of Deep Eutectic Solvent on the Extraction and in Vitro Antioxidant Activity of Rutin from Sophora Japonica Bud. Journal of Food Science and Technology, 55, 2326-2333. [Google Scholar] [CrossRef] [PubMed]
[12] Liu, Y., Zhe, W., Zhang, R., Peng, Z., Wang, Y., Gao, H., et al. (2022) Ultrasonic-Assisted Extraction of Polyphenolic Compounds from Paederia scandens (Lour.) Merr. Using Deep Eutectic Solvent: Optimization, Identification, and Comparison with Traditional Methods. Ultrasonics Sonochemistry, 86, Article ID: 106005. [Google Scholar] [CrossRef] [PubMed]
[13] 李晓珺, 曹龙辉, 刘慧燕, 等. 深共晶溶剂结合超声波辅助提取南非叶总黄酮工艺优化及其抗氧化活性研究[J]. 食品科技, 2025, 50(4): 247-256.
[14] 王柏强, 项静, 张杰, 等. 超声波技术辅助酶解法提取杜仲叶总黄酮工艺优化[J]. 中国药业, 2021, 30(2): 28-30.
[15] 宋佳敏, 王鸿飞, 孙朦, 等. 响应面法优化金蝉花多糖提取工艺及抗氧化活性分析[J]. 食品科学, 2018, 39(4): 275-281.