枸杞多糖结合电针对鱼藤酮诱导帕金森病大鼠神经元保护作用研究
Research on the Protective Effect of Lycium barbarum Polysaccharides Combined with Electroacupuncture on Neurons of Parkinson’s Disease Rats Induced by Rotenone
DOI: 10.12677/acm.2025.15102930, PDF,   
作者: 赵纯冰*：石河子大学第一附属医院药学部，新疆 石河子；任罗艺：石河子大学第一附属医院介入中心，新疆 石河子；李抒遥：石河子大学第一附属医院眼科，新疆 石河子；史军辉：石河子大学临床医学院，新疆 石河子
关键词: 帕金森病；枸杞多糖；电针；Parkinson’s Disease； Lycium barbarum Polysaccharides； Electroacupuncture
摘要: 目的：观察枸杞多糖(LBP)结合电针对帕金森病(PD)模型大鼠黑质环氧合酶-2 (COX-2)、肿瘤坏死因子(TNF-α)和酪氨酸羟化酶(TH)表达的影响，探究LBP结合电针在帕金森病治疗中的可能作用机制。方法：30只SD大鼠，随机分为：正常组、模型组、LBP组、电针组和结合组，各6只。使用鱼藤酮于颈背部皮下注射制备PD动物模型，药物组进行LBP灌胃；电针组采用高频电针(100 Hz)治疗；结合组先给予LBP灌胃，再行电针治疗；三组均治疗21天。通过免疫组化检测黑质COX-2、TH、TNF-α的表达。结果：PD大鼠黑质COX-2表达增加，LBP、电针和结合治疗都可使黑质COX-2表达降低(P < 0.01)，但结合治疗效果优于单用LBP或电针(P < 0.05)。PD大鼠黑质TNF-α表达增加，LBP、电针和结合治疗都可使黑质TNF-α表达降低(P < 0.01)，但结合治疗效果优于单用LBP或电针(P < 0.05)。PD大鼠黑质TH表达减少，LBP、电针和结合治疗都可使黑质TH表达增加(P < 0.01)，但结合治疗效果优于单用LBP或电针(P < 0.05)。结论：LBP结合电针治疗PD大鼠可有效保护PD模型大鼠的多巴胺能神经元，且作用强于单用LBP或电针。
Abstract: Objective: To observe the effects of Lycium barbarum polysaccharides (LBP) combined with electroacupuncture on the expression of substantia nigra cyclooxygenase-2 (COX-2), tumor necrosis factor (TNF-α) and tyrosine hydroxylase (TH) in Parkinson’s disease (PD) model rats, and to explore the possible mechanism of LBP combined with electroacupuncture in the treatment of Parkinson’s disease. Methods: Thirty SD rats were randomly divided into normal group, model group, LBP group, electroacupuncture group and combined group, with 6 rats in each group. Animal models of PD were prepared by subcutaneous injection of rotenone in the neck and back, and LBP gavage was performed in the drug group. The electroacupuncture group was treated with high-frequency electroacupuncture (100 Hz). The combination group was given LBP gavage first, followed by electroacupuncture. All three groups were treated for 21 days. The expression of substantia nigra COX-2, TH, TNF-α was detected by immunohistochemistry. Results: The expression of COX-2 in the substantia nigra rats was increased, and the expression of COX-2 in the substantia nigra was decreased by LBP, electroacupuncture and combination therapy (P < 0.01), but the effect of combination therapy was better than that of LBP alone or electroacupuncture (P < 0.05). The expression of TNF-α in substantia nigra rats was increased, and the expression of TNF-α in substantia nigra was decreased by LBP, electroacupuncture and combination therapy (P < 0.01), but the effect of combined treatment was better than that of LBP alone or electroacupuncture (P < 0.05). The expression of substantia nigra TH was decreased, and LBP, electroacupuncture and combination therapy could increase the expression of substantia nigra TH (P < 0.01), but the effect of combination treatment was better than that of LBP alone or electroacupuncture (P < 0.05). Conclusion: LBP combined with electroacupuncture can effectively protect dopaminergic neurons in PD model rats, and the effect is stronger than that of LBP or electroacupuncture alone.
文章引用：赵纯冰, 任罗艺, 李抒遥, 史军辉. 枸杞多糖结合电针对鱼藤酮诱导帕金森病大鼠神经元保护作用研究[J]. 临床医学进展, 2025, 15(10): 1651-1658. https://doi.org/10.12677/acm.2025.15102930

参考文献

[1] Tolosa, E., Garrido, A., Scholz, S.W. and Poewe, W. (2021) Challenges in the Diagnosis of Parkinson’s Disease. The Lancet Neurology, 20, 385-397. [Google Scholar] [CrossRef] [PubMed]
[2] Xie, C., Zhang, Y., Wang, X., Chen, J., Chen, Y., Pa, J., et al. (2015) Levodopa Alone Compared with Levodopa-Sparing Therapy as Initial Treatment for Parkinson’s Disease: A Meta-Analysis. Neurological Sciences, 36, 1319-1329. [Google Scholar] [CrossRef] [PubMed]
[3] Raja, K., Ramrakhia, S., Dev, K., Shahid, W., Sohail, H., Memon, M.K., et al. (2020) The Risk Factors for the Wearing-Off Phenomenon in Parkinson’s Disease. Cureus, 12, e10729. [Google Scholar] [CrossRef] [PubMed]
[4] 宋鲁平, 王强. 帕金森病康复中国专家共识[J]. 中国康复理论与实践, 2018, 24(7): 745-752.
[5] 陈浩, 张皓洁, 师亮, 等. 枸杞多糖对帕金森病小鼠的抗氧化作用和神经保护效应[J]. 中国神经精神疾病杂志, 2018, 44(10): 613-618.
[6] Teismann, P., Vila, M., Choi, D., Tieu, K., Wu, D.C., Jackson‐Lewis, V., et al. (2003) COX‐2 and Neurodegeneration in Parkinson’s Disease. Annals of the New York Academy of Sciences, 991, 272-277. [Google Scholar] [CrossRef] [PubMed]
[7] 陈忻, 张楠, 赵晖, 等. 鱼藤酮致帕金森病大鼠行为学与黑质病理损伤的关系[J]. 中国神经精神疾病杂志, 2008, 34(4): 232-234.
[8] Yang, L., Zhou, R., Tong, Y., Chen, P., Shen, Y., Miao, S., et al. (2020) Neuroprotection by Dihydrotestosterone in LPS-Induced Neuroinflammation. Neurobiology of Disease, 140, Article ID: 104814. [Google Scholar] [CrossRef] [PubMed]
[9] Rauf, A., Badoni, H., Abu-Izneid, T., Olatunde, A., Rahman, M.M., Painuli, S., et al. (2022) Neuroinflammatory Markers: Key Indicators in the Pathology of Neurodegenerative Diseases. Molecules, 27, Article No. 3194. [Google Scholar] [CrossRef] [PubMed]
[10] Liu, N., Bai, L., Lu, Z., Gu, R., Zhao, D., Yan, F., et al. (2022) TRPV4 Contributes to ER Stress and Inflammation: Implications for Parkinson’s Disease. Journal of Neuroinflammation, 19, Article No. 26. [Google Scholar] [CrossRef] [PubMed]
[11] Hunot, S. and Hirsch, E.C. (2003) Neuroinflammatory Processes in Parkinson’s Disease. Annals of Neurology, 53, S49-S60. [Google Scholar] [CrossRef] [PubMed]
[12] Pradhan, S.S., Salinas, K., Garduno, A.C., Johansson, J.U., Wang, Q., Manning-Bog, A., et al. (2016) Anti-Inflammatory and Neuroprotective Effects of PGE2 EP4 Signaling in Models of Parkinson’s Disease. Journal of Neuroimmune Pharmacology, 12, 292-304. [Google Scholar] [CrossRef] [PubMed]
[13] Liang, X., Wu, L., Wang, Q., Hand, T., Bilak, M., McCullough, L., et al. (2007) Function of COX-2 and Prostaglandins in Neurological Disease. Journal of Molecular Neuroscience, 33, 94-99. [Google Scholar] [CrossRef] [PubMed]
[14] Xiromerisiou, G., Marogianni, C., Lampropoulos, I.C., Dardiotis, E., Speletas, M., Ntavaroukas, P., et al. (2022) Peripheral Inflammatory Markers TNF-α and CCL2 Revisited: Association with Parkinson’s Disease Severity. International Journal of Molecular Sciences, 24, Article No. 264. [Google Scholar] [CrossRef] [PubMed]
[15] Jiang, X., Wang, X., Tuo, M., Ma, J. and Xie, A. (2018) RAGE and Its Emerging Role in the Pathogenesis of Parkinson’s Disease. Neuroscience Letters, 672, 65-69. [Google Scholar] [CrossRef] [PubMed]
[16] Singh, S.B., Braun, C.A., Carroll-Portillo, A., Coffman, C.N. and Lin, H.C. (2024) Sulfate-Reducing Bacteria Induce Pro-Inflammatory TNF-α and iNOS via PI3K/Akt Pathway in a TLR 2-Dependent Manner. Microorganisms, 12, Article No. 1833. [Google Scholar] [CrossRef] [PubMed]
[17] 贾思玮, 郭敏芳, 王记委, 等. 枸杞多糖治疗神经退行性疾病的研究进展[J]. 中国老年学杂志, 2025, 45(16): 4077-4082.
[18] Gao, K., Liu, M., Cao, J., Yao, M., Lu, Y., Li, J., et al. (2014) Protective Effects of Lycium barbarum Polysaccharide on 6-OHDA-Induced Apoptosis in PC12 Cells through the ROS-NO Pathway. Molecules, 20, 293-308. [Google Scholar] [CrossRef] [PubMed]
[19] Song, J., Liu, L., Li, Z., Mao, T., Zhang, J., Zhou, L., et al. (2022) Lycium barbarum Polysaccharide Improves Dopamine Metabolism and Symptoms in an MPTP-Induced Model of Parkinson’s Disease. BMC Medicine, 20, Article No. 412. [Google Scholar] [CrossRef] [PubMed]
[20] Yang, Y., Li, W., Li, Y., Wang, Q., Gao, L. and Zhao, J. (2014) Dietary Lycium barbarum Polysaccharide Induces Nrf2/ARE Pathway and Ameliorates Insulin Resistance Induced by High-Fat via Activation of PI3K/AKT Signaling. Oxidative Medicine and Cellular Longevity, 2014, Article ID: 145641. [Google Scholar] [CrossRef] [PubMed]
[21] 王顺, 戚秀杰, 韩迪. 头部电针透穴疗法对帕金森病模型大鼠黑质TH及DAT基因表达的影响[J]. 中国针灸, 2009, 29(5): 391-394.
[22] 胡梦妮, 马骏, 魏佳勇, 等. 电针对帕金森病小鼠中脑黑质胰岛素信号转导的影响[J/OL]. 针刺研究: 1-8. https://link.cnki.net/doi/10.13702/j.1000-0607.20241069, 2025-08-30.[CrossRef] 
[23] 王晓平, 罗永杰, 吴景芬, 等. 多巴丝肼联合电针对老年帕金森病患者血清白细胞介素-1β、胱抑素C及血清离子水平的影响[J]. 中国老年学杂志, 2016, 36(17): 4301-4303.
[24] 贾多, 唐祎泽, 张佳, 等. 电针联合五子衍宗丸通过调节BDNF/TrkB/CREB信号通路对帕金森病小鼠的治疗作用[J]. 世界中医药, 2024, 19(14): 2098-2105.

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