植入前无创胚胎选择技术：从培养基中提取的miRNA到AI的多维评估
Non-Invasive Preimplantation Embryo Selection: From Culture Medium miRNA to Multidimensional Evaluation with AI
DOI: 10.12677/acm.2025.15102959, PDF,   
作者: 陈芷若*, 农 夏, 彭丽思, 王玉霞#：暨南大学附属第一医院妇产科，广东 广州
关键词: 辅助生殖技术；无创；胚胎选择；ART； Non-Invasive； Embryo Selection
摘要: 辅助生殖技术(ART)为不孕夫妇带来新的希望，但胚胎选择方法仍是影响妊娠结局的关键因素。本文综述了无创胚胎选择的主要研究进展，包括培养基中的miRNA与cfDNA检测、延时成像(TLI)和人工智能(AI)分析。传统形态学评估和PGT-A虽被广泛应用，但分别存在主观性强与侵入性高等局限。相比之下，无创技术在保障胚胎安全的同时，能够从分子、生物学及形态学多维度提供更客观的评估。当前证据表明，这些方法在提高胚胎筛选准确性、缩短妊娠周期和提升活产率方面具有潜力。然而技术标准化不足、结果一致性差及成本效益不明仍是推广的主要障碍。未来研究应聚焦多模态数据融合与大规模临床验证，以推动无创技术在ART中的临床转化，并为个体化胚胎选择提供可靠依据。
Abstract: Assisted reproductive technology (ART) has brought new hope to infertile couples, yet embryo selection remains a critical factor influencing pregnancy outcomes. This review summarizes recent advances in non-invasive embryo selection, including microRNA (miRNA) and cell-free DNA (cfDNA) detection in spent culture media, time-lapse imaging (TLI), and artificial intelligence (AI)-based analysis. Conventional approaches, such as morphological assessment and preimplantation genetic testing for aneuploidy (PGT-A), are widely used but limited by subjectivity and invasiveness, respectively. In contrast, non-invasive techniques ensure embryo safety while providing multidimensional evaluation from molecular, biological, and morphokinetic perspectives. Current evidence suggests their potential to improve selection accuracy, shorten time to pregnancy, and increase live birth rates. However, lack of technical standardization, inconsistent reproducibility, and unclear cost-effectiveness remain major barriers to clinical application. Future research should focus on multimodal data integration and large-scale clinical validation to promote the translation of non-invasive technologies in ART and support individualized embryo selection strategies.
文章引用：陈芷若, 农夏, 彭丽思, 王玉霞. 植入前无创胚胎选择技术：从培养基中提取的miRNA到AI的多维评估[J]. 临床医学进展, 2025, 15(10): 1885-1893. https://doi.org/10.12677/acm.2025.15102959

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