可理解组合语义的大肠杆菌重编程方法
Programming Escherichia coli to Understand Compositional Syntax
DOI: 10.12677/hjbm.2025.156117, PDF,   
作者: 陈 梅：中央民族大学信息工程学院，北京
关键词: DNA计算；DNA存储；基因电路；CRISPR/Cas9；DNA Computing； DNA Storage； Genetic Circuit； CRISPR/Cas9
摘要: 理解组合语义在人类交流中至关重要。本研究开发出一种能够编程活细胞的策略，利用CRISPR/Cas9系统的精准基因组编辑能力来解析组合语义。在我们的实验中，大肠杆菌成功区分了“good morning”与“morning good”。这项研究不仅证明基因组编辑技术可用于存储或记录信息，更能用于识别信息。通过该策略，人类与活细胞的通信范围将拓展至任何可编码为DNA的信息，这将推动智能遗传器件的构建。我们相信该策略在生物计算、生物传感、生物治疗等领域具有广阔的应用前景。
Abstract: Understanding compositional syntax is important in human communication. Here, we developed a strategy which can program living cells to understand compositional syntax through precise genome editing ability of CRISPR/Cas9 system. In our example, the E. coli successfully tells the difference between “good morning” and “morning good”. This study shows not only can genome editing technology be used in storing or recording information, but it can also be used in understanding information. Through this strategy, the range that people can communicate with living cells will widen to any information which can be encoded into DNA. This will advance the construction of intelligent genetic devices. We believe that this strategy has many potential applications in biocomputing, biosensing, biotherapy, and so on.
文章引用：陈梅. 可理解组合语义的大肠杆菌重编程方法[J]. 生物医学, 2025, 15(6): 1087-1094. https://doi.org/10.12677/hjbm.2025.156117

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