南方红豆杉8个NAC低温响应转录因子的生物信息学分析

Bioinformatic Analysis of Eight NAC Cold-Resistant Transcription Factors in Taxus chinensis var. mairei

摘要: 为揭示南方红豆杉NAC抗逆因子的分子机制提供理论依据，本研究以南方红豆杉的转录组测序数据为基础，利用生物信息学方法，对8条响应冷胁迫表达量高的南方红豆杉NAC蛋白序列的氨基酸组成、理化性质、系统进化、motif分析、亚细胞定位以及二级和三级结构开展预测和分析。研究结果表明：8个NAC转录因子的氨基酸数目差距较大，但在疏水性和稳定方面差异不大；二级结构预测中，8条氨基酸序列的结构主要由α-螺旋、β-转角与无规则卷曲组成。三级结构中各个结构均有差异，但是部分序列三维结构相似。系统进化树和蛋白互作分析结果进一步验证了转录组测序中关于转录因子功能注释的可靠性。本研究结果为南方红豆杉NAC转录因子的进一步研究提供了理论依据。

Abstract: This study provides a theoretical basis for revealing the molecular mechanism of stress resistance factors of NAC in southern Taxus chinensis var. Mairei. Based on transcriptome sequencing data of southern Taxus chinensis var. Mairei, bioinformatics methods were used to predict and analyze the amino acid composition, physicochemical properties, phylogeny, motif analysis, subcellular localization, and secondary and tertiary structures of eight NAC protein sequences with high expression levels in response to cold stress. The research results indicate that there is a significant difference in the number of amino acids among the 8 NAC transcription factors, but there is not much difference in hydrophobicity and stability; in secondary structure prediction, the structure of the 8 amino acid sequences is mainly composed of alpha helix and beta angle and random coil. Each structure in the tertiary structure has differences, but some sequences have similar three-dimensional structures. The results of the phylogenetic tree and protein interaction analysis further validated the consistency of transcription factor functional annotations in transcriptome sequencing. The results of this study provide a theoretical basis for further research on NAC transcription factors in southern Taxus chinensis var. Mairei.