转Ht1-FFT基因油菜的获得及其耐旱性分析
The Obtaining of Transgenic Rapeseed with Ht1-FFT Gene and Its Drought Tolerance Analysis
DOI: 10.12677/hjas.2025.1511166, PDF,    国家自然科学基金支持
作者: 宋楚媛, 梁玲娇, 刘卓彤, 刘春林, 阮 颖*：作物表观遗传调控与发育湖南省重点实验室，湖南农业大学，湖南 长沙；岳麓山实验室，湖南 长沙
关键词: Ht1-FFT；果聚糖；油菜；耐旱；Ht1-FFT； Fructan； Brassica napus； Drought Tolerance
摘要: 随着油菜种植区域向北大面积扩展，苗期旱害成为了油菜生产上面临的重要挑战。本研究探讨了果聚糖合成酶基因Ht1-FFT对增强植株抗旱性的作用。通过遗传转化将基因导入油菜材料湘油15 (XY15)中，筛选获得6个阳性植株，经分子鉴定与表达分析证实，该外源基因Ht1-FFT在转基因植株中实现了稳定整合和有效过表达。在干旱条件下，转基因植株抗旱能力显著提高，其体内积累更高的果聚糖，果聚糖积累量与耐旱性之间均呈正相关；同时脯氨酸含量、叶片相对含水量、相对电导率及丙二醛等膜稳定性生理胁迫指标也得到改善，这些说明Ht1-FFT的表达有效增强了转基因油菜的抗旱能力，这为油菜耐旱育种提供了新的方法与新的种质资源。
Abstract: With the large-scale northward expansion of rapeseed cultivation, drought stress at the seedling stage has become a major challenge in rapeseed production. This study investigates the role of the fructan synthase gene Ht1-FFT in enhancing drought tolerance in plants. Through genetic transformation, the gene was introduced into the rapeseed cultivar Xiangyou 15 (XY15), and six positive transgenic plants were selected and identified. Molecular identification and expression analysis confirmed the stable integration and effective overexpression of the exogenous gene Ht1-FFT in the transgenic plants. Under drought conditions, the transgenic plants exhibited significantly improved drought resistance, accompanied by higher fructan accumulation. A positive correlation was observed between fructan accumulation and drought tolerance. Additionally, physiological stress indicators related to membrane stability, including proline content, leaf relative water content, relative electrical conductivity, and malondialdehyde content, were also improved. These results demonstrate that the expression of Ht1-FFT effectively enhances drought tolerance in transgenic rapeseed, providing a new approach and germplasm resources for drought-tolerant rapeseed breeding.
文章引用：宋楚媛, 梁玲娇, 刘卓彤, 刘春林, 阮颖. 转Ht1-FFT基因油菜的获得及其耐旱性分析[J]. 农业科学, 2025, 15(11): 1319-1328. https://doi.org/10.12677/hjas.2025.1511166

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