J、K亚型禽白血病病毒双重PCR检测方法建立及应用

Establishment and Application of Multiple PCR for Detection of Avian Leukosis Viruses Subgroup J and K

摘要: 为检测进境鸡群是否感染禽白血病病毒(Avian leukosis virus, ALV)，本研究针对主要的外源性ALV亚群ALV-J和ALV-K的基因保守区域设计引物和探针，通过体系优化，特异性、灵敏性和重复性评估，建立了可同时检测ALV-J和ALV-K的双重荧光PCR方法。结果显示，该方法与禽多种疫病无交叉反应，有较好的线性关系，ALV-J和ALV-K亚型的检测限分别为3.27 copies/μL和3.28 copies/μL。利用本研究建立的方法与传统的ELISA方法进行比对，结果表明建立的双重PCR方法检出率更高，可用于口岸禽白血病监测和疫病防控。

Abstract: In order to detect Avian leukosis virus (ALV) infection in imported chickens, primers and probes were designed for the gene-conserved regions of major exogenous ALV subpopulations ALV-J and ALV-K. Through system optimization, specificity, sensitivity and reproducibility evaluation, primers and probes were designed. A dual fluorescence PCR method was developed to detect ALV-J and ALV-K simultaneously. The results showed that there was no cross-reaction between the method and various avian diseases, and there was a good linear relationship with the detection limit of 3.27 copies/μL and 3.28 copies/μL. Compared with the traditional ELISA method, the results showed that the established dual PCR method had a higher detection rate, and could be used for avian leukemia monitoring and disease prevention at ports.