PBL联合翻转课堂教学法在微生物学实验课程中的应用——以动植物检疫专业为例

Application of a PBL Combined with Flipped Classroom Teaching Method in a Microbiology Laboratory Course—A Case Study of the Plant and Animal Quarantine Major

摘要: 为满足应用型动植物检疫人才培养目标，切实提高学生的实践能力。本文基于PBL结合翻转课堂混合式教学法，从解决“腹泻仔猪病原微生物分离、鉴定与耐药性检测”这一生产实际问题出发，将培养基的配制，微生物的分离、纯化，革兰氏染色，菌种保藏，细菌的生化试验，细菌的药物敏感性试验等常规微生物学实验有机整合。一方面强化实验技能的学习和应用，另一方面结合翻转课堂教学模式，进一步增强学习的积极性和主动性，最终达到了“知识–能力–素质”协同培养的人才培养目标。

Abstract: To meet the talent cultivation objectives of applied plant and animal quarantine professionals and effectively enhance students’ practical abilities, this study adopts a blended teaching approach integrating Problem-Based Learning (PBL) and the flipped classroom model. Based on addressing the practical production issue of “isolation, identification, and drug resistance testing of pathogenic microorganisms in diarrheic piglets”, conventional microbiology experiments—such as medium preparation, microbial isolation and purification, Gram staining, strain preservation, biochemical tests of bacteria, and bacterial drug sensitivity tests—were systematically integrated. This approach not only strengthens the learning and application of experimental skills but also, combined with the flipped classroom model, further enhances students’ enthusiasm and initiative in learning. Ultimately, it achieves the talent cultivation goal of synergistic development in “knowledge, ability, and quality”.