VIGS技术引入分子生物学实验教学实践

Practice of VIGS Technology in Molecular Biology Experiment Teaching

摘要: 随着分子生物学技术的快速发展，传统分子生物学实验教学中实验设计碎片化、验证性实验占比过高、前沿技术和实际应用融入不足等问题日益凸显。本研究将病毒诱导的基因沉默(Virus-Induced Gene Silencing, VIGS)技术引入分子生物学实验教学，设计了以烟草脆裂病毒(TRV)为载体、番茄为模式植物、SlPDS基因功能为研究目标的实验体系。通过构建VIGS载体、农杆菌介导的植物接种、表型观察及基因表达分析等实验环节，使学生掌握基因功能研究的核心分子生物学技术。通过教学实践，提升学生的分子操作技能和科研思维能力，为高校分子生物学实验教学改革提供借鉴。

Abstract: With the rapid advancement of molecular biology techniques, the limitations of traditional molecular biology experimental teaching have become increasingly apparent. These include fragmented experimental designs, an overreliance on verification-based experiments, and a lack of integration between cutting-edge technologies and practical applications. This study integrates Virus-Induced Gene Silencing (VIGS) technology into the molecular biology laboratory curriculum by designing an experimental framework that utilizes the Tobacco rattle virus (TRV) as a vector, tomato as the model plant, and the functional analysis of the SlPDS gene as the research focus. Through a series of experimental modules-such as VIGS vector construction, Agrobacterium-mediated plant transformation, phenotypic observation, and gene expression analysis-students gain mastery of core molecular biology techniques used in gene function research. This teaching approach effectively improves students’ molecular operational skills and scientific research abilities, offering valuable insights for reforming molecular biology experimental teaching in higher education institutions.