基于HS-SPME-GC-MS法分析化橘红中的挥发油成分

Analysis of Volatile Oil Components in Exocarpium Citri grandis Using HS-SPME-GC-MS Method

摘要: 探究化橘红中挥发性成分的种类、相对含量。利用固相微萃取(SPME)两种不同涂层材料，但萃取效果类同的萃取头结合气相色谱–质谱联用技术(GC-MS)，对化橘红挥发油进行成分分析，研究发现相对含量最高的三个成分：β-月桂烯、D-柠檬烯与大根香叶烯D。使用两种萃取效果类同的萃取头50/30 μm (DVB/CAR/PDMS 50/30 um)和萃取头65 μm (PDMS)主要是为了确保最后结果的有效性、真实性、稳定性及准确性。固相微萃取的样品前处理技术不需要溶剂，避免了传统提取方法中溶剂残留对分析结果的影响，同时也减少了试验过程中对环境的污染。由于其具有简单、快速、灵敏度高、易于自动化等优点，在化橘红挥发油成分提取中展现出独特优势，提高挥发油的质量和产量，在挥发油成分分析中显示出巨大的潜力。

Abstract: This study investigates the types and relative contents of volatile components in Exocarpium Citri grandis. By utilizing solid-phase microextraction (SPME) with two different coating materials, but with similar extraction efficacy, combined with gas chromatography-mass spectrometry (GC-MS), the volatile oil components of Exocarpium Citri grandis were analyzed. The study found that the three components with the highest relative content were β-laurolene, D-limonene, and D-germacrene. The use of two SPME fibers with similar extraction performance, 50/30 μm (DVB/CAR/PDMS 50/30 μm) and 65 μm (PDMS), was primarily to ensure the validity, authenticity, stability, and accuracy of the final results. The sample pretreatment technique of solid-phase microextraction does not require solvents, thereby avoiding the impact of solvent residues on analytical results commonly seen in traditional extraction methods, while also reducing environmental pollution during the experiment. Due to its simplicity, speed, high sensitivity, and ease of automation, SPME demonstrates a unique advantage in the extraction of volatile oil components of Exocarpium Citri grandis, enhancing both the quality and yield of the essential oil, and showing great potential in volatile oil component analysis.