通过常温常压等离子体技术(ARTP)选育高产阿维链霉菌菌株

Selection of High-Yield Streptomyces avermitilis Strains through Ambient Temperature and Pressure Plasma Technology

摘要: 阿维链霉菌是工业上生产阿维菌素的重要菌株，其发酵效率高低关系到企业生产的效益。本研究采用常温常压等离子体(ARTP)技术对阿维链霉菌进行诱变选育，首次诱变设置10 s~120 s共9个时间梯度，分析不同处理对菌株致死率的影响，在该区间内进一步缩小时间梯度范围(40 s、45 s、50 s、55 s、60 s)进行诱变，并结合摇瓶发酵筛选高产突变株。结果显示，经45 s诱变处理的突变株阿维菌素效价达8.85 g/L，较原始菌株提升15.4%，研究证实ARTP技术可通过诱变菌体并进一步筛选获得产率较高的突变株，本研究为阿维菌素工业化生产构建了高效、可行的育种新策略。

Abstract: Streptomyces avermitilis is an important strain for industrial production of Avermectin, and its fermentation efficiency is closely related to the production benefits of enterprises. In this study, the ARTP (Atmospheric and Room Temperature Plasma) technology was used to mutagenize and select Streptomyces avermitilis. The first mutagenesis was set with 9 time gradients from 10 s to 120 s to analyze the effect of different treatments on the mortality rate of the strain. Within this range, the time gradient was further narrowed (40 s, 45 s, 50 s, 55 s, 60 s) for mutagenesis, and high-yield mutant strains were screened by shake flask fermentation. The results showed that the Avermectin titer of the mutant strain treated with 45 s mutagenesis reached 8.85 g/L, which was 15.4% higher than that of the original strain. The study confirmed that the ARTP technology can obtain mutant strains with higher production rates by mutagenizing the bacteria and further screening, This study has established a highly efficient and feasible new breeding strategy for the industrial production of Abamectin.