泌尿系大肠埃希菌质粒携带特征与耐药表型关联性分析

Analysis of the Correlation between Plasmid Carrying Characteristics and Drug Resistance Phenotype of Uropathogenic Escherichia coli

摘要: 目的：探究泌尿系感染来源的84株大肠埃希菌质粒携带特征，分析质粒类型与抗菌药物耐药表型的关联性，为临床耐药防控和抗菌药物选择提供参考。方法：采用全基因组测序技术鉴定84株大肠埃希菌的质粒类型，通过BD Phoenix 100微生物全自动鉴定药敏仪检测菌株对6类常用抗菌药物的耐药性，统计分析质粒携带与耐药表型的关联。结果：84株大肠埃希菌均携带质粒，多质粒(≥3个)携带率为89.29% (75/84)；优势质粒类型为IncF家族(85.71%, 72/84)、Col质粒(50%, 42/84)和IncI质粒(17.86%, 15/84)。菌株多重耐药率(75.00%, 63/84)；IncF质粒与头孢菌素类、喹诺酮类耐药呈显著正相关(<0.001)。结论：泌尿系大肠埃希菌IncF家族为优势质粒类型，不同质粒类型与特定抗菌药物耐药存在靶向关联，可作为临床耐药防控的重要靶点。

Abstract: Objective: To explore the plasmid carrying characteristics of 84 strains of Escherichia coli from urinary tract infection, and analyze the correlation between plasmid types and antimicrobial resistance phenotype, so as to provide reference for clinical drug resistance prevention and control and antimicrobial drug selection. Methods: The plasmid types of 84 strains of Escherichia coli were identified by genome-wide sequencing technology, and the drug resistance of the strains to six kinds of commonly used antibacterial drugs was detected by BD Phoenix 100 microbial automatic drug sensitivity analyzer, and the relationship between plasmid carrying and drug resistance phenotype was statistically analyzed. Results: 84 strains of Escherichia coli all carried plasmids, and the carrying rate of multiple plasmids (≥3) was 89.29% (75/84). The dominant plasmid types are IncF family (85.71%, 72/84), Col plasmid (50%, 42/84) and IncI plasmid (17.86%, 15/84). The multidrug resistance rate of the strain was 75.00% (63/84). IncF plasmid was positively correlated with cephalosporins and quinolones resistance (< 0.001). Conclusion: IncF family of urinary Escherichia coli is the dominant plasmid type, and different plasmid types have targeted correlation with specific antimicrobial resistance, which can be used as an important target for clinical drug resistance prevention and control.