基于网络药理学探讨前泌通片对治疗慢性前列腺炎的分子机制

Exploring the Molecular Mechanism of Qianmitong Tablets in the Treatment of Chronic Prostatitis Based on Network Pharmacology

摘要: 本研究利用网络药理学和分子对接方法，系统探讨了中成药前泌通片(QMT)治疗慢性前列腺炎(CP)的潜在分子机制。研究人员首先从TCMSP等数据库中筛选出QMT的活性成分及其作用靶点，并从GeneCards等数据库中收集慢性前列腺炎相关的疾病靶点。通过取交集获得药物与疾病的共同靶点，构建了蛋白相互作用(PPI)网络并筛选出核心靶点(如MAPK3, FOS, AKT1等)。随后，通过GO和KEGG富集分析揭示了这些靶点主要参与的生物学过程和信号通路，如VEGF信号通路、细胞凋亡和代谢通路等。最后，通过分子对接技术验证了柚皮素、槲皮素等主要活性成分与核心靶点之间具有良好的结合能力。研究结论认为，前泌通片通过多成分、多靶点、多通路的方式，在抑制血管生成、调节细胞凋亡、纠正代谢紊乱等方面发挥作用，从而治疗慢性前列腺炎。

Abstract: This study systematically investigated the potential molecular mechanism of the Chinese patent medicine Qianmitong Tablet (QMT) in the treatment of chronic prostatitis (CP) using network pharmacology and molecular docking methods. Firstly, researchers screened the active ingredients of QMT and their corresponding targets from databases such as TCMSP, and collected CP-related disease targets from databases including GeneCards. The common targets between the drug and the disease were obtained by taking the intersection, after which a protein-protein interaction (PPI) network was constructed and core targets (such as MAPK3, FOS, AKT1, etc.) were screened out. Subsequently, GO and KEGG enrichment analyses revealed the biological processes and signaling pathways mainly involved in these targets, including the VEGF signaling pathway, apoptosis, and metabolic pathways. Finally, molecular docking technology verified that major active ingredients such as naringenin and quercetin had good binding abilities to the core targets. The study concluded that Qianmitong Tablet exerts therapeutic effects on chronic prostatitis through a multi-component, multi-target, and multi-pathway mode, by inhibiting angiogenesis, regulating apoptosis, and correcting metabolic disorders.