杠板归提取物对小鼠急性肺损伤的作用

Therapeutic Effects of Extract from Persicaria Perfoliata on Acute Lung Injury in Mice

摘要: 探讨杠板归提取物对脂多糖(LPS)诱导的急性肺损伤(ALI)小鼠模型的抗炎作用。实验设置空白组、LPS组(5 g/kg)、LPS (5 g/kg) + 杠板归组(20 g/(kg·d))。在LPS干预之前，给予杠板归提取物干预小鼠7天，随后，采用LPS构建ALI小鼠模型。LPS干预12小时后，处死小鼠，获取小鼠肺组织及血清，通过HE染色观察肺组织病理变化，ELISA法检测血清中IL-6炎症因子水平。我们的结果显示，与LPS组相比，杠板归提取物组小鼠肺组织病理损伤减轻，血清中IL-6炎症因子水平显著降低(P < 0.05)。因此，我们的研究表明，杠板归提取物能够缓解ALI病理损伤，减轻肺部炎症反应。

Abstract: To Explore the anti-inflammatory effect of persicaria perfoliata extract on lipopolysaccharide (LPS)-Induced acute lung injury (ALI) in a mouse model, the experimental groups included a blank control group, an LPS group (5 g/kg), and an LPS (5 g/kg) + persicaria perfoliata extract group (20 g/(kg·d)). Before LPS administration, the mice were pretreated with persicaria perfoliata extract for 7 days, followed by the induction of ALI using LPS. Twelve hours after LPS intervention, the mice were euthanized, and lung tissues and serum were collected. Pathological changes in lung tissue were observed via HE staining, and serum IL-6 levels were measured using ELISA. Our results showed that compared with the LPS group, the general condition of the mice in the persicaria perfoliata extract group improved, pathological lung injury was alleviated, and serum IL-6 levels were significantly reduced (P < 0.05). Therefore, our study demonstrates that persicaria perfoliata extract can mitigate pathological damage in ALI and reduce pulmonary inflammatory responses.