不同理化因子对枇杷(Eriobotrya japonica (Thunb.) L.)花粉体外萌发和花粉管生长的影响

Effects of the Different Factors on Pollen Germination and Pollen Tube Growth in Eriobotrya japonica(Thunb.) L.

摘要: 本实验以枇杷花粉为材料，采用花粉离体培养法研究了蔗糖浓度、pH值以及γ-氨基丁酸(GABA)对枇杷花粉萌发和花粉管生长的影响。试验以31 mg/L H₃BO₃、196.8 mg/L MgSO₄•7H₂O、7.45 mg/L KCl + 56 mg/L CaCl₂为基本培养基组分，在25℃暗条件下培养5 h后统计枇杷花粉萌发率及花粉管的生长速率。结果表明，蔗糖浓度为10%时，花粉萌发率达到最高值60%，同时花粉管生长速率达到最大值70.4 µm/h；pH 8.0时花粉萌发率最高，达63%；此外，添加不同浓度的GABA对花粉萌发的影响不显著，但对花粉管生长的影响有显著作用。

Abstract: In this study, loquat fresh pollens were used as materials to explore the effect of sucrose concen-tration, pH and gamma-amino butyric acid (GABA) on pollen germination and pollen tube growth in vitro. The basic culture medium consists with 31 mg/L boric acid + 196.8 mg/L MgSOIn this study, loquat fresh pollens were used as materials to explore the effect of sucrose concen-tration, pH and gamma-amino butyric acid (GABA) on pollen germination and pollen tube growth in vitro. The basic culture medium consists of 31 mg/L boric acid + 196.8 mg/L MgSO₄•7H₂O + 7.45 mg/L KCl + 56 mg/L CaCl₂. Pollens were cultured in dark at 25°C for 5 h. The results showed that pollen germination rate reached to 60% and pollen tube growth rate was 70.4 µm/h. Given 10% sucrose and pH value 8.0, pollen germination reached the highest ratio of 63%. Moreover, the ad-dition of GABA to the medium can effectively promote the growth rate of pollen tubes.for 5 h. The results showed that pollen germination rate reached to 60% and pollen tube growth rate was 70.4 µm/h. Given 10% sucrose and pH value 8.0, pollen germination reached the highest ratio of 63%. Moreover, the addition of GABA to the medium can effectively promote the growth rate of pollen tubes.