红水河草鱼线粒体D-Loop和Cyt b基因序列的对比分析

Comparison of Mitochondrial D-Loop and Cyt b Sequences of Ctenopharyngodon idellus

摘要: 研究了红水河野生草鱼Ctenopharyngodon idellus上游、中游和下游3个群体29个个体的线粒体控制区(D-loop)和细胞色素b (Cyt b)基因序列，通过PCR扩增与测序，分别获得了长度为846 bp和1083 bp的D-loop和Cyt b基因片段。分析表明，D-loop序列共定义了9个单倍型，存在10个多态位点，发生转换4次，插入/缺失6次。Cyt b序列共定义了9个单倍型，存在9个多态性位点，发生转换1次，插入/缺失8次。两序列分析结果表明，群体间和群体内遗传距离较小，遗传变异的分子变异等级不显著。上、中、下游均有共享单倍型，3个群体间遗传分化不大。

Abstract: Sequences of mitochondrial D-loop and Cyt b gene of 24 individuals of Ctenopharyngodon idellus which were sampled from three populations in Hongshui River were determined. Using PCR am-plification and sequencing, 846 bp of D-loop partial sequences and 1083 bp of Cyt b partial se-quences were obtained. In D-loop sequences, a total of 10 polymorphic sites were defined; 9 distinct haplotypes, 4 transitions, and 6 insert/lacuna loci were found. In Cyt b sequences, 9 polymorphic sites and 9 haplotypes were defined; 1 transition and 8 insert/lacuna loci were found. Two-sequence analysis showed that, within groups and between groups, the genetic distance is small, and the genetic variation of molecular variation level is not significant. Upper, middle and lower reaches are shared haplotypes, and the genetic differentiation among three populations is little.