野生白芨种苗快繁与驯化试验研究

Experimental Research on Rapid Propagation and Domestication of Wild Bletilla Seedling

摘要: 对野生白芨进行组培快繁研究，结果表明：1) MS + 2.5 mg∙L⁻¹6-BA + 0.2 mg∙L⁻¹ NAA诱导效果最好，诱导率可达98%；2) MS + 2.5 mg∙L⁻¹ IAA + 2.5 mg∙L⁻¹6-BA + 2.0 mg∙L⁻¹ NAA诱导增殖的效果最好，培养40 d后增殖率可达3.8倍；3) 1/2 MS + 0.01 mg∙L⁻¹ NAA +0.01 mg∙L⁻¹ IAA有利于生根，且植株长势健壮。炼苗基质以60%腐叶土 + 30%珍珠岩 + 10％蛭石为佳；炼苗温度23℃~28℃，空气相对湿度80%~90%有利于提高炼苗成活率。

Abstract: Tissue culture and rapid propagation research was done on wild bletilla. The results showed that: 1) MS + 2.5 mg∙L⁻¹6-BA + 0.2 mg∙L⁻¹ NAA has the best induced effect, whose induction rate can reach 98%; 2) MS + IAA 2.5 mg∙L⁻¹ + 2.5 mg∙L⁻¹6-BA +2.0 mg∙L⁻¹ NAA has the best effect of induced proliferation, whose proliferation rate can be up to 3.8 times after 40 d culture; 3) 1/2 MS + 0.01 mg∙L⁻¹ NAA + 0.01 mg∙L⁻¹ IAA is conducive to take root and plants grow strong. The best rotten leaves seedling substrate consists of 60% leaf mold + 30% perlite + 10% vermiculite. The seedling survival rate can be improved in temperature of 23˚C - 28˚C and air relative humidity of 80% - 90%.