基于微流控CL芯片的厚朴药材提取物的抗氧化活性分析

Magnolia officinalis Extract Antioxidant Activity Analysis Based on Microfluidic CL Chip

摘要: 设计并制作了一种集成有四个重复结构单元的微流控化学发光(chemiluminescence, CL)芯片，以实现中药材厚朴提取物的分子水平的抗氧化活性分析。芯片结构单元包含蛇形通道、高效环形混合通道和椭圆形检测区。微流控CL芯片系统一次可完成单一样本多浓度的检测。采用luminol-H₂O₂-Cu²⁺化学发光体系对厚朴酚、和厚朴酚标品进行CL测试以验证该芯片可靠性，并得出最佳测试条件为：Luninol浓度为0.50 mmol∙L⁻¹，H₂O₂浓度为1.00 mmol∙L⁻¹，Cu²⁺浓度为0.50 mmol∙L⁻¹，流速10.0 µL∙min⁻¹。进而对厚朴药材五种醇提物进行CL测试，结果表明，五种提取物对H₂O₂均有一定的清除作用，且1.00 g∙ml⁻¹的五种提取物的当量抗氧化活性强弱顺序为：80%乙醇提取物 > 60%乙醇提取物 > 40%乙醇提取物 > 20%乙醇提取物 > 水提取物。

Abstract: An integrated microfluidic chip with four repeating structural units was designed and fabricated to achieve molecular level of Magnolia medicines extracts antioxidant activity analysis. Chip structure unit includes a serpentine channel, efficient mixing channel and an oval ring detection zone. The Microfluidic chemiluminescence chip system could successfully complete synchronous detection of single sample with different concentration. Using luminol-H₂O₂-Cu²⁺chemiluminescence system for Magnolol, Honokiol CL-standard products were tested to verify the reliability of the chip, and optimum test conditions are: luminol concentration is 0.50 mmol∙L⁻¹, the H₂O₂ concentration is 1.00 mmol∙L⁻¹, Cu²⁺ concentration is 0.50 mmol∙L⁻¹ and the flow rate is 10.0 μL∙min⁻¹. Furthermore Magnolia officinalis extracts from five alcohol antioxidant activity test results showed that the five extracts antioxidant activity, which was extracted under different condition from 1.00 g∙mL⁻¹ Magnolia officinalis, has a certain effect to remove H₂O₂, and the order of antioxidant activity strength was: 80% ethanol extract > 60% ethanol extract > 40% ethanol extract> 20% ethanol extract> aqueous extract.